Now showing items 101-110 of 810
Synthesis and Purification of Oligonucleotide N3′→︀P5′ Phosphoramidates and their Phosphodiester and Phosphorothioate Chimeras
(Wiley Periodicals, Inc., 2000-12)
This unit describes the synthesis and purification of oligonucleotide N3′→︀P5′ phosphoramidates, wherein each 3′‐oxygen is replaced by a 3′‐amine in the 2′‐deoxyribose ring. The synthesis of required monomers and application ...
Postnatal development of beta-endorphin immunoreactivity in the medulla oblongata of rat
(Elsevier, 1985-02)
Beta-endorphin (B-END) like immunoreactivity (i.r.) levels were measured by radioimmunoassay in the medulla oblongata of developing rats on postnatal ages P1-P42 at 7 day intervals, and in adult rats. From P1 to P42, B-END ...
Effect of dinitrophenol on the interaction between myosin and nucleotides
(Elsevier, 1959-04)
1. 1. The effects of 2,4-dinitrophenol (DNP) on the interaction of several nucleoside triphosphates with myosin B and glycerol-treated psoas fibers have been studied at 0.3 M KCl, 10-3 M Ca++, pH 7.0, 25.5 [deg]C.2. 2. The ...
Dopamine [beta]-hydroxylation in diabetes and diabetic autonomic neuropathy
(Elsevier, 1981-08)
Dopamine [beta]-hydroxylase (DBH) has been studied in healthy subjects and diabetic patients, some of whom had autonomic neuropathy. DBH was assayed by a recently described in vivo tritium release method (10,11). Dopamine ...
Direct optical resolution of vesamicol and a series of benzovesamicol analogues by high-performance liquid chromatography
(Elsevier, 1994-04-29)
The direct optical resolution of the vesicular acetylcholine uptake inhibitors vesamicol and benzovesamicol and nine benzovesamicol analogues were performed by HPLC on a commercially available cellulose tris(3,5-dimethylphenyl ...
Site‐Specific Fluorescent Labeling of Large RNAs with Pyrene
(Oxford University PressWiley Periodicals, Inc., 2004-12)
Pyrene is a useful chromophore for monitoring the tertiary structure and folding of large RNAs. This unit describes the general preparation of a large RNA (>80 nucleotides in length) that has been site‐specifically modified ...
The purification and properties of an alcohol dehydrogenase from mouse liver
(Elsevier, 1974-04)
1. 1. An alcohol dehydrogenase (alcohol: NAD oxidoreductase, EC ( [middle dot] [middle dot] ) was isolated from the supernatant fraction of mouse liver and purified 206-fold.2. 2. A number of aliphatic aldehydes, ranging ...
Preparation of 2′‐O‐[(Triisopropylsilyl)oxy]methyl‐protected Ribonucleosides
(Oxford University PressWiley Periodicals, Inc., 2001-12)
The [(triisopropylsilyl)oxy]methyl (TOM) group is a useful protecting group for the 2′‐OH of ribonucleosides to be used for oligoribonucleotide synthesis by the phosphoramidite method. It is completely stable to all reaction ...
Modulation of opioid signal transduction in SH-SY5Y neural cells by differentiating agents: Concurrent mu receptor upregulation and effector desensitization by phorbol ester
(Elsevier, 1994-02-21)
Long-term exposure of SH-SY5Y human neural cells to retinoic acid (RA) increased the binding of [3H]DAMGO and [3H]DPDPE by up to 3-fold compared to membranes of untreated cells. In contrast, incubation of the cells with ...