Now showing items 121-130 of 810
Protection of 5′‐Hydroxy Functions of Nucleosides
(IRL PressWiley Periodicals, Inc., 2000-02)
The 5‐hydroxy group is the primary hydroxy group of nucleosides. It is mandatory to protect 5‐hydroxyls in all methods of oligonucleotide synthesis that require nucleoside synthons. This unit discusses a wide variety of ...
Simple, rapid, and highly efficient separation of amino acid phenylthiohydantoins by reversed-phase high-performance liquid chromatography
(Elsevier, 1982-04)
A rapid and efficient separation of amino acid phenylthiohydantoins by high-performance liquid chromatography has been accomplished by step-gradient elution with use of a mobile phase of acetate-buffered aqueous acetonitrile ...
Purification of the pancreatic cholecystokinin receptor
(Elsevier, 1989-03)
We have previously shown that the pancreatic cholecystokinin (CCK) receptor can be solubilized in 1% digitonin. In this study, digitonin-solubilized CCK receptors from rat pancreas were purified using sequential affinity ...
Glycolytic activity in extracts of young rat skin
(Elsevier, 1959-01)
1. 1. Data have been presented which indicate the presence of most of the glycolytic enzymes in the skin of the young rat.2. 2. A cell-free extract of this tissue will stoichiometrically convert fructose diphosphate to ...
Electron paramagnetic resonance studies on nitrogenase : I. The properties of molybdoferredoxin and azoferredoxin
(Elsevier, 1972-11)
Both molybdoferredoxin and azoferredoxin exhibit EPR signals. Reduced azoferredoxin has a g = 1.94 type signal characteristic of nonheme iron proteins. Reduced molybdoferredoxin has EPR signals with g-values of 2.01, 3.78 ...
Slouching toward a systems approach to treatment
(Elsevier, 1994)
Both the health care sector generally and the alcohol and drug field are moving toward a truly systematic approach to treatment, accelerated by the anticipated advent of health care reform. There is considerable reluctance ...
Detection of electrophoretically silent mutations by immobilized pH gradients
(Elsevier, 1986)
The detection of neutral amino acid mutants, by isoelectric focusing in immobilized pH gradients (IPGs), is exemplified by the separation of hemoglobin (Hb) Beirut (126[beta] Val --> Ala) from Hb A in a shallow pH 7.2-7.6 ...
Characterization of a mutant human erythrocyte carbonic anhydrase: Carbonic anhydrase IcGuam: The amino acid substitution and carboxylesterase and hydratase activities
(Elsevier, 1966-11)
Evidence is presented which indicates that an arginine residue has substituted for a glycine residue in the abnormal human erythrocyte carbonic anhydrase, CA IcGuam. The ratio of the amount of mutant to normal enzyme ...