Now showing items 91-100 of 169
Attachment of Nucleosides to Solid‐Phase Supports
(ESCOMWiley Periodicals, Inc., 2000-02)
Specific step‐by‐step instructions are given for coupling nucleosides to LCAA‐CPG supports (supports consisting of a long‐chain alkylamine linked to controlled‐pore glass). Protocols are given for a succinic acid linker ...
Metabolomics revealed the toxicity of cationic liposomes in HepG2 cells using UHPLC‐Q‐TOF/MS and multivariate data analysis
(Wiley Periodicals, Inc., 2017-12)
Cationic liposomes (CLs) are novel nonviral vectors widely used for delivering drugs or genes. However, applications of CLs are largely hampered by their cytotoxicity, partly because the potential mechanism underlying the ...
Synthesis of Oligonucleotide Conjugates via Aqueous Diels‐Alder Cycloaddition
(Wiley Periodicals, Inc.Cold Spring Harbor Laboratory Press, 2003-09)
The conjugation of maleimide reporter groups to 5′‐diene‐modified oligonucleotides via aqueous Diels‐Alder cycloaddition is described. Detailed basic protocols are provided for the preparation of a diene‐amidite (5′‐diene ...
Synthesis and Polymerase Incorporation of 5′‐Amino‐2′,5′‐Dideoxy‐5′‐N‐Triphosphate Nucleotides
(Wiley Periodicals, Inc., 2004-09)
This unit presents synthetic procedures for the preparation of 5′‐amino‐2′,5′‐dideoxy analogs of adenosine, cytidine, guanosine, and thymidine, as well as corresponding 5′‐N‐triphosphate nucleotides, using commercially ...
Direct Attachment of Conjugate Groups to the 5′ Terminus of Oligodeoxyribonucleotides
(Wiley Periodicals, Inc., 2000-02)
This unit gives protocols for the attachment of intercalating and photoreactive conjugate groups to oligodeoxyribonucleotides. Protocols are given for acridine‐ and psoralen‐conjugated oligonucleotides, and include attachment ...
Determination of Binding Thermodynamics
(Wiley Periodicals, Inc.JAI Press, 2000-02)
This unit serves as a starting point for exploring the thermodynamic properties of interactions between small molecules and DNA. It covers the determination of simple, apparent association/dissociation constants. The ...