Now showing items 31-40 of 169
Palladium‐Mediated C5 Substitution of Pyrimidine Nucleosides
(Wiley Periodicals, Inc.IRL Press, 2000-02)
One of the most efficient ways to link a reporter group to oligonucleotides is through the incorporation of a modified nucleoside during automated oligonucleotide synthesis. To be useful, it is important that the reporter ...
Unnatural Nucleosides with Unusual Base Pairing Properties
(John Wiley & Sons, 2001-07)
Synthetic modified nucleosides designed to pair in unusual ways with natural nucleobases have many potential applications in nucleic acid biochemistry. This overview lays the foundation for future protocol units on synthesis ...
EPR analysis of multiple forms of [4Fe–4S] 3+ clusters in HiPIPs
(Springer-Verlag; SBIC, 2005-06)
The electron paramagnetic resonance (EPR) spectrum from the [4Fe–4S] 3+ cluster in several high-potential iron–sulfur proteins (HiPIPs) is complex: it is not the pattern of a single, isolated S =1/2 system. Multifrequency ...
Nucleoside and Nucleotide Nomenclature
(Wiley Periodicals, Inc., 2000-02)
Current nomenclature in the area of nucleosides, nucleotides, and nucleic acids comprises a mixture of (1) common names that have gained official recognition, (2) guidelines that have been derived and officially recommended ...
A UPLCâ MS/MS method for simultaneous determination of five flavonoids from Stellera chamaejasme L. in rat plasma and its application to a pharmacokinetic study
(Wiley Periodicals, Inc., 2018-06)
Stellera chamaejasme L. has been used as a traditional Chinese medicine for the treatment of scabies, tinea, stubborn skin ulcers, chronic tracheitis, cancer and tuberculosis. A sensitive and selective ultraâ high liquid ...
Characteristics of Nucleic Acids
(Wiley Periodicals, Inc.Cold Spring Harbor Laboratory Press, 2000-02)
This appendix is a compilation of information from Current Protocols in Molecular Biology (John Wiley & Sons, Inc.) and provides information about the major nucleoside/nucleotide constituents and structures for A‐, B‐, and ...
Copper-lowering therapy with tetrathiomolybdate for cancer and diseases of fibrosis and inflammation The University of Michigan has recently licensed the antiangiogenic uses of TM to Attenuon LLC, and Dr. Brewer has equity in Attenuon LLC
(Wiley Subscription Services, Inc., A Wiley Company, 2003)
Angiogenesis is required for tumor growth and is a likely Achilles heel for cancer. However, antiangiogenic agents have been somewhat disappointing in cancer therapy, perhaps because they target a single angiogenic factor, ...
Synthesis and Purification of Peptide Nucleic Acids
(Wiley Periodicals, Inc., 2002-06)
Peptide nucleic acids (PNAs) are DNA analogs in which the normal phosphodiester backbone is replaced by 2‐aminoethyl glycine linkages. Hybridization of PNAs with RNA or DNA follows normal rules for Watson‐Crick base pairing ...
Probing DNA Structure with Hydroxyl Radicals
(Wiley Periodicals, Inc., 2001-12)
The hydroxyl radical is a useful probe for studying the shape of the surface of a DNA molecule. Using this technique, fine details of DNA structure can potentially be revealed. This unit describes how to use the hydroxyl ...
Engineering Terminal Disulfide Bonds Into DNA
(Wiley Periodicals, Inc.Springer‐Verlag, 2003-06)
This protocol presents a simple and general means of modifying nucleic acids with disulfide cross‐links. These cross‐links serve as powerful tools to probe the structure, dynamics, thermodynamics, folding, and function of ...