Now showing items 721-730 of 811
Analysis and Purification of Synthetic Nucleic Acids Using HPLC
(Wiley Periodicals, Inc.Stockton Press, 2000-07)
HPLC is a powerful and popular method for analyzing and purifying biomolecules. Reversed‐phase HPLC allows a high‐capacity method for purification, and uses volatile buffer systems that simplify product recovery. Anion‐exchange ...
A turn in the road: How studies on the pharmacology of glucosylceramide synthase inhibitors led to the identification of a lysosomal phospholipase A 2 with ceramide transacylase activity
(Kluwer Academic Publishers; Springer Science+Business Media, 2003-01)
A series of inhibitors of glucosylceramide synthesis, the PDMP based family of compounds, has been developed as a tool for the study of sphingolipid biochemistry and biology. During the course of developing more active ...
Effect of 2-N-mono- and 2-N-diethylaminoethanol on normal and choline-deficient rats
(Elsevier, 1967)
In rats, the acute oral LD50 value of neutralized 2-N-monoethylaminoethanol (MEAE) was found to be 1.0 (0.68-1.35) g/kg. The acute oral toxicity of MEAE, but not that of 2-N-diethylaminoethanol (DEAE) could be largely ...
Two-dimensional peptide mapping by reversed-phase column chromatography, applied to the sequence determination of cytochrome c from the wild type and a mutant of the butterfly, pieris brassicae
(Elsevier, 1983-08-26)
Two-dimensional peptide mapping has been very effective in the characterization of protein digests, particularly for the detection of small structural differences between homologous proteins. The classical thin-layer ...
[beta]-Endorphin suppression of acute morphine abstinence in morphine dependent monkeys: Effective given intraventricularly but ineffective given intravenously
(Elsevier, 1985-07)
Six female adult monkeys were made dependent upon morphine sulfate and were implanted with a chronic indwelling needle in the lateral ventricle of the brain for sterile intraventricular injections. Both [beta]-endorphin ...
Prodynorphin peptide immunocytochemistry in rhesus monkey brain
(Elsevier, 1985)
The present study describes the immunocytochemical distribution of peptides derived from the prodynorphin precursor in the brain of the rhesus monkey (Macaca mulatta). Animals were treated with colchicine (intracerebrove ...
Epimerization of trans-4-hydroxy--proline to cis-4-hydroxy--proline during acid hydrolysis of collagen
(Elsevier, 1972-10)
1. 1. trans-4-Hydroxy--proline is epimerized to cis-4-hydroxy--proline under the conditions generally used for the hydrolysis of proteins for analyses of amino acids.2. 2. The cis epimer elutes from a column of UR-30 resin ...
Evidence that removal of an endogenous metal that stabilizes the untransformed glucocorticoid receptor in cytosol allows ligand-independent receptor transformation
(Elsevier, 1989)
Cytosol preparations contain an endogenous heat-stable factor which stabilizes the glucocorticoid receptor in its untransformed, non DNA-binding form. Elution of a partially purified preparation of this stabilizing factor ...
Stereoselective high-performance liquid chromatographic assay for pirmenol enantiomers in dog plasma
(Elsevier, 1991-12-15)
Pirmenol enantiomers in dog plasma were quantified using a stereospecific high-performance liquid chromatographic method with ultraviolet detection at 262 nm. Racemic pirmenol and internal standard, (+)-propranolol, were ...