Now showing items 81-90 of 336
Protein extraction using the sodium bis(2-ethylhexyl) phosphate (NaDEHP) reverse micellar system
(Wiley Subscription Services, Inc., A Wiley Company, 1996-04-20)
The reverse micellar system of sodium bis(2-ethylhexyl) phosphate (NaDEHP)/isooctane/brine was used for liquid-liquid extraction of proteins. We investigated the solubilization of cytochrome-c and Α-chymotrypsin into the ...
Protamine immobilization and heparin adsorption on the protamine-bound cellulose fiber membrane
(Wiley Subscription Services, Inc., A Wiley Company, 1992-02-20)
Immobilization of protamine to the inner lumen of cellulose hollow fibers has been shown useful in preventing both heparin- and protamine-induced complications during an extracorporeal blood circulation procedure. The ...
Determining the extent of labeling for tetramethylrhodamine protein conjugates
(Elsevier, 1991-10-25)
A new, relatively simple, spectrophotometric technique has been developed which is useful for accurately determining the extent of chromophore labeling of proteins. Often the absorbance spectra and extinction coefficients ...
A rapid attenuation of muscarinic agonist stimulated phosphoinositide hydrolysis precedes receptor sequestration in human SH-SY-5Y neuroblastoma cells
(Kluwer Academic Publishers-Plenum Publishers; Plenum Publishing Corporation ; Springer Science+Business Media, 1994-05)
Agonist occupancy of muscarinic cholinergic receptors in human SH-SY-5Y neuroblastoma cells elicited two kinetically distinct phases of phosphoinositide hydrolysis when monitored by either an increased mass of inositol ...
Irreversible enzyme-shuttle immunoassay
(Wiley Subscription Services, Inc., A Wiley Company, 1992-03-25)
The concept of a competitive enzyme immunoassay that utilizes simultaneously the bound and the free analyte-enzyme conjugate (heterobifunctional conjugate) for signal generation in response to varying analyte concentrations ...
Repeated haloperidol increases both calmodulin and a calmodulin-binding protein in rat striatum
(Elsevier, 1994-12)
Repeated treatment with the antipsychotic drug, haloperidol, leads to an increased behavioral sensitivity to dopamine agonists exhibited upon withdrawal from the drug. An increase in the particulate content of the endogenous ...
Glycine-extended post-translational processing intermediates of gastrin and cholecystokinin in the gut
(Elsevier, 1994-02-03)
Cholecystokinin (CCK) and gastrin are two polypeptide hormones of the gut that share complete structural homology in their carboxyl-terminal pentapeptide. Both peptides are biologically activated from their glycine-extended ...
Inhibitors of thymidylate synthase and dihydrofolate reductase potentiate the antiviral effect of acyclovir
(Elsevier, 1993-03)
In cells infected with herpes simplex virus type 1, intracellular dNTP pools increased markedly. Treatment of these cells with 3 [mu]M acyclovir resulted in an additional expansion in pyrimidine deoxyribonucleoside ...
Degradation kinetics of pentachlorophenol by Phanerochaete chrysosporium
(Wiley Subscription Services, Inc., A Wiley Company, 1990-05)
The extracellular enzymes and cell mass from the pregrown Phanerochaete chrysosporium cultures were used for the degradation of PCP. The use of both extracellular enzymes and cell mass resulted in extensive mineralization ...