Now showing items 41211-41220 of 41314
A Neuron-Specific Histone Demethylase Complex Fine Tunes Neurodevelopment
(2022)
Cell-type specific transcriptional programs are thought to be defined by cell-type specific transcription factors (TFs) that bind to specific DNA sequences. In contrast, chromatin regulators are ubiquitously expressed and ...
Droplet Microfluidics Coupled to Capillary Electrophoresis and Mass Spectrometry for Pharmacological Applications
(2022)
Modern drug discovery relies on high-throughput screening for lead generation and protein target evaluation. There is a critical need to develop assays that not only have large-scale screening capabilities but provide ...
Low Temperature Electrodeposition of Epitaxial Silicon Thin Films
(2022)
This thesis explores the applicability of Si electrodeposition to the synthesis of crystalline Si at low temperatures. Electrodeposition has been long perceived as an ideal route for the synthesis of semiconductor materials ...
Understanding, Communicating, and Reducing Analytical Uncertainty: Theory, Visualization Designs, and an Augmented Presentation System to Support Validation and Interpretation of a Multiverse Analysis
(2024)
Analyzing data is a complex, multi-step process, with multiple choices available at each step, such as whether and how to exclude outliers, what approach to use to operationalize a variable, or what model and parameters ...
Evolutionary Constraints and Potential of the Influenza A Virus RNA-dependent RNA Polymerase
(2023)
Due to rapid evolution and adaptation, influenza viruses remain a major health concern despite co-existing with human beings for centuries. The influenza virus polymerase is a major driver of influenza virus evolution. ...
Characterization and Improvement of a Nicotine-Degrading Flavoenzyme
(2024)
Nicotine oxidoreductase (NicA2) is a flavin-dependent enzyme that has the ability to catalyze oxidation of nicotine to N-methylmyosmine. Though nicotine is a psychoactive substance with high addictive potential, N-methylmyosmine ...
Structure and Targeting of Precursor MicroRNA-31: From Mechanism to Application
(2024)
As an essential post-transcriptional regulator of gene expression, microRNA (miRNA) levels must be strictly maintained. The biogenesis of many miRNAs is regulated by trans-acting protein partners, which exert control through ...
Investigating the Regulatory Pathways of the Mitotic Oscillator Via a High-Throughput Droplet-Based System
(2024)
This dissertation aims to investigate the properties of the mitotic oscillator and its complex regulatory mechanism. Responsible for the progression of the cell cycle through its various stages, the mitotic oscillator is ...
Development and Validation of Transportable, Clinically Applicable and Scalable Machine Learning Models for Acute Kidney Injury
(2024)
Acute kidney injury (AKI), a frequent complication in hospitalized patients, poses significant challenges due to its high incidence, short-term mortality, and substantial economic burden. Current AKI models utilizing ...
The Measurement and Optimization of Direct Laser Acceleration
(2023)
This thesis presents experimental measurements and numerical modeling of electron acceleration optimization and laser channel formation in relativistic laser interactions with underdense plasma, as well as scintillator ...