Now showing items 31-40 of 93
Loss of antibody productivity is highly reproducible in multiple hybridoma subclones
(Wiley Subscription Services, Inc., A Wiley Company, 1993-06-20)
An immunoglobulin G (IgG 2b ) producing hybridoma cell line (S3H5/Γ2bA2) was cloned and subcloned. Twenty subclones were grown in parallel while being adapted in a stepwise fashion to serum-free medium. Following adaptation ...
The rate and locus of development of rat vallate taste buds
(Elsevier, 1991-02-22)
Several hundred taste buds develop and mature in the trench walls of the rat's vallate papilla during the first 3 months after birth. The objective of this investigation of taste bud development was to determine: (i) whether ...
Differential expression of metabotropic glutamate receptors in the hippocampus and entorhinal cortex of the rat
(Elsevier, 1994-02)
Metabotropic glutamate receptors (mGluRs) have been implicated in a number of hippocampal functions including learning and memory. Five subtypes have been molecularly and pharmacologically characterized. Using in situ ...
Countercurrent gradient chromatography: A continuous focusing technique
(Wiley Subscription Services, Inc., A Wiley Company, 1995-12-05)
The continuous separation of proteins was performed in a countercurrent gradient chromatography (CGC) system. A magnetically stabilized fluidized bed (MSFB) was used to establish true countercurrent contact of a solid resin ...
Membrane adsorption characteristics determine the kinetics of flow-through transductions
(Wiley Subscription Services, Inc., A Wiley Company, 1996-08-05)
Retrovirus-mediated gene transfer is currently limited by random Brownian motion of the retrovirus. This limitation can be overcome by flowing the retrovirus solution through a porous membrane that supports the target ...
Corticotropin releasing hormone is involved in exercise-induced elevation in core temperature
(Elsevier, 1994)
To determine the involvement of corticotropin releasing factor (CRF) in exercise-induced elevation in core temperature of female rats, CRF antibody or vehicle was injected intracerebroventricularly (ICV) into rats that had ...
Whole skeletal muscle transplantation: Mechanisms responsible for functional deficits
(Wiley Subscription Services, Inc., A Wiley Company, 1994-04-05)
One aspect of tissue engineering of skeletal muscle involves the transposition and transplantation of whole muscles to treat muscles damaged by injury or disease. The transposition of whole muscles has been used for many ...
Expression of Corticotropin-Releasing Hormone Transgenes in Neurons of Adult and Developing Mice
(Elsevier, 1994-12)
The DNA sequences important for cell-specific expression and developmental regulation of corticotropin-releasing hormone (CRH) were analyzed in transgenic mice. A construct containing 0.5 kb of CRH 5' flanking DNA linked ...
Immobilization can improve the stability of hybridoma antibody productivity in serum-free media
(Wiley Subscription Services, Inc., A Wiley Company, 1990-12-05)
Hybridoma cells (S3H5/Γ2bA2) were cultivated in spinner flasks with 1% serum media and serum-free media. Monoclonal antibody productivity was maintained in 1% serum media. However, cells in serum-free media showed a decrease ...
Formation of polyol–fatty acid esters by lipases in reverse micellar media
(Wiley Subscription Services, Inc., A Wiley Company, 1992-06-05)
The synthesis of polyol–fatty acid esters has strong implications in such industries as foods, cosmetics, and polymers. We have investigated these esterification reactions employing the polyols ethylene glycol, 2-monoglyceride, ...