Now showing items 1-10 of 18
Rapid protein release from Escherichia coli by chemical permeabilization under fermentation conditions
(Wiley Subscription Services, Inc., A Wiley Company, 1992-03-25)
Overall protein release greater than 75% in less than 1 h can be attained by exposing exponentially growing Escherichia coli cells to 0.4 M guanidine plus 0.5% Triton X-100 at 37°C in medium. Cell growth stops immediately ...
The effects of menstrual phase and nicotine abstinence on nicotine intake and on biochemical and subjective measures in women smokers: a A preliminary report
(Elsevier, 1992-11)
Nicotine intake, menstrual and smoking withdrawal symptomatology, and baseline cortisol and MHPG were assessed in nine women smokers under conditions of ad lib smoking and overnight abstinence in three menstrual phases ...
Gene expression of the insulin-like growth factors and their receptors in cultured human retinal pigment epithelial cells
(Elsevier, 1992-01)
Insulin-like growth factors I and II (IGF I and II) are polypeptides with both growth-promoting and insulin-like metabolic effects16,27. Immunoreactive IGF I is present in the retina14 and both IGF I and II are present in ...
Population balance between producing and nonproducing hybridoma clones is very sensitive to serum level, state of inoculum, and medium composition
(Wiley Subscription Services, Inc., A Wiley Company, 1992-02-05)
Secreting and nonsecreting hybridoma populations derived from the murine hybridoma cell line 167.4G5.3 were each grown in batch culture in low serum and serum-free media. Under serum-free conditions, a secreting population ...
Gene expression of the insulin-like growth factors and their receptors in human neuroblastoma cell lines
(Elsevier, 1992-10)
Insulin-like growth factors (IGF) I and II are polypeptides with both growth-promoting and insulin-like metabolic effects. The developmentally specific expression of IGF I and II in the nervous system implies a role for ...
Tissue-specific expression of the human neuropeptide Y gene in transgenic mice
(Elsevier, 1992-06)
Neuropeptide Y (NPY) is the most abundant neuropeptide detected in the mammalian brain, and is found throughout the central and peripheral nervous systems. This peptide is a proposed regulator of appetite, blood pressure, ...
Protamine immobilization and heparin adsorption on the protamine-bound cellulose fiber membrane
(Wiley Subscription Services, Inc., A Wiley Company, 1992-02-20)
Immobilization of protamine to the inner lumen of cellulose hollow fibers has been shown useful in preventing both heparin- and protamine-induced complications during an extracorporeal blood circulation procedure. The ...
Irreversible enzyme-shuttle immunoassay
(Wiley Subscription Services, Inc., A Wiley Company, 1992-03-25)
The concept of a competitive enzyme immunoassay that utilizes simultaneously the bound and the free analyte-enzyme conjugate (heterobifunctional conjugate) for signal generation in response to varying analyte concentrations ...
Effects of ammonia and lactate on hybridoma growth, metabolism, and antibody production
(Wiley Subscription Services, Inc., A Wiley Company, 1992-02-20)
The influence of ammonia and lactate on cell growth, metabolic, and antibody production rates was investigated for murine hybridoma cell line 163.4G5.3 during batch culture. The specific growth rate was reduced by one-half ...
Formation of polyol–fatty acid esters by lipases in reverse micellar media
(Wiley Subscription Services, Inc., A Wiley Company, 1992-06-05)
The synthesis of polyol–fatty acid esters has strong implications in such industries as foods, cosmetics, and polymers. We have investigated these esterification reactions employing the polyols ethylene glycol, 2-monoglyceride, ...