Now showing items 1-10 of 27
An Antibiotic Discovery Campaign Targeting VirF, the Main Transcriptional Regulator of Virulence in Shigella flexneri
(2016)
Shigella flexneri is a gram-negative enteropathogen that infects the human colonic epithelium. It is estimated that Shigella spp. infect 165 million people a year worldwide. Symptoms of shigellosis include bloody dysentery, ...
Vibrational Probe and Methods Development for Studying the Ultrafast Dynamics of Preferential Solvation of Biomolecules by 2D-IR.
(2015)
Over the last decade two-dimensional infrared spectroscopy (2D-IR) has emerged as a powerful method for the investigation of biological samples and their dynamics. Through the implementation of state of the art signal ...
A NMR Investigation of Structure and Allostery in the Hsp70 Chaperone System.
(2010)
The Hsp70 Chaperone system is a complex molecular machine composed of proteins. It is responsible for assisting in protein folding/refolding/processing and transport. Hsp70 proteins have been historically associated with ...
Disruption of Endosomal Membrane by Cationic Vectors Drives Endosomal Release and Enables Successful Gene Delivery
(2016)
The potential of gene therapy to treat congenital disorders is hindered by the lack of safe and effective delivery agents (vectors). More effective viral vectors possess several safety concerns but the safer non-viral ...
Targeting the BCL9/B9L Binding Interaction with B-catenin as a Potential Anticancer Strategy.
(2010)
Wnt signaling plays a critical role in numerous cellular processes including embryonic development, cell proliferation and tissue homeostasis. The multifunctional protein β-catenin is the primary mediator of canonical Wnt ...
The Application of Mass Spectrometry-Based Label-Free Quantitative Proteomic Strategies in Cancer Stem Cell Research.
(2011)
The application of mass spectrometry(MS) has dramatically expanded the frontiers of the analysis of protein expression in biological samples. This thesis addresses the application of a MS-based label-free strategy to analyze ...
Exploring a "Nature-Inspired" Strategy for Enhancing the Pharmacology of Small Molecules: Towards the Development of Next Generation Anti-Infectives.
(2011)
HIV is now a pandemic of staggering proportions. Unfortunately, the current generation of antiretroviral therapeutics is only partially and temporarily effective in suppressing viral replication due to rapid metabolism ...
Mapping, Tracking and Modeling the Movements of Single Membrane-Bound Transcription Activator Proteins in Live Vibrio cholerae.
(2014)
Cholera has been known since ancient times, yet there is still no cure. Vibrio cholerae bacteria cause cholera by producing cholera toxin. Cholera toxin production is regulated by the protein ToxT, which itself is regulated ...
Folate Binding Protein as a Therapeutic Natural Nanotechnology
(2018)
Serum proteins interact with small molecules and nanoparticles in blood, resulting in protein coronas. Protein coronas influence the bioidentity of the molecules and nanoparticles, playing critical roles in biotransport, ...
(1) The Relationship of Protein Expression and Cell Division, (2) 3D Imaging of Cells Using Digital Holography, and (3) General Chemistry Enrollment at University of Michigan.
(2012)
Chapter 1: We evaluated the relationship between cell division and protein expression when using commercial poly(ethylenimine) (PEI)-based polyplexes. The membrane dye PKH26 was used to assess cell division, and cyan ...