Human Transgene-Free Amniotic-Fluid-Derived Induced Pluripotent Stem Cells for Autologous Cell Therapy
dc.contributor.author | Jiang, Guihua | |
dc.contributor.author | Di Bernardo, Julie | |
dc.contributor.author | Maiden, Michael M. | |
dc.contributor.author | Villa-Diaz, Luis G. | |
dc.contributor.author | Mabrouk, Omar S. | |
dc.contributor.author | Krebsbach, Paul H. | |
dc.contributor.author | O'Shea, K. Sue | |
dc.contributor.author | Kunisaki, Shaun M. | |
dc.date.accessioned | 2017-12-19T21:15:38Z | |
dc.date.available | 2017-12-19T21:15:38Z | |
dc.date.issued | 2014-07-11 | |
dc.identifier.citation | Jiang, Guihua; Di Bernardo, Julie; Maiden, Michael M.; Villa-Diaz, Luis G.; Mabrouk, Omar S.; Krebsbach, Paul H.; O'Shea, K. Sue; Kunisaki, Shaun M. (2014). "Human Transgene-Free Amniotic-Fluid-Derived Induced Pluripotent Stem Cells for Autologous Cell Therapy." Stem Cells and Development 23 (21): 2613-2625. | |
dc.identifier.issn | 1547-3287 | |
dc.identifier.uri | https://hdl.handle.net/2027.42/140204 | |
dc.description.abstract | The establishment of a reliable prenatal source of autologous, transgene-free progenitor cells has enormous potential in the development of regenerative-medicine-based therapies for infants born with devastating birth defects. Here, we show that a largely CD117-negative population of human amniotic fluid mesenchymal stromal cells (AF-MSCs) obtained from fetuses with or without prenatally diagnosed anomalies are readily abundant and have limited baseline differentiation potential when compared with bone-marrow-derived MSCs and other somatic cell types. Nonetheless, the AF-MSCs could be easily reprogrammed into induced pluripotent stem cells (iPSCs) using nonintegrating Sendai viral vectors encoding for OCT4, SOX2, KLF4, and cMYC. The iPSCs were virtually indistinguishable from human embryonic stem cells in multiple assays and could be used to generate a relatively homogeneous population of neural progenitors, expressing PAX6, SOX2, SOX3, Musashi-1, and PSA-NCAM, for potential use in neurologic diseases. Further, these neural progenitors showed engraftment potential in vivo and were capable of differentiating into mature neurons and astrocytes in vitro. This study demonstrates the usefulness of AF-MSCs as an excellent source for the generation of human transgene-free iPSCs ideally suited for autologous perinatal regenerative medicine applications. | |
dc.publisher | Mary Ann Liebert, Inc., publishers | |
dc.title | Human Transgene-Free Amniotic-Fluid-Derived Induced Pluripotent Stem Cells for Autologous Cell Therapy | |
dc.type | Article | |
dc.subject.hlbtoplevel | Health Sciences | |
dc.description.peerreviewed | Peer Reviewed | |
dc.description.bitstreamurl | https://deepblue.lib.umich.edu/bitstream/2027.42/140204/1/scd.2014.0110.pdf | |
dc.identifier.doi | 10.1089/scd.2014.0110 | |
dc.identifier.source | Stem Cells and Development | |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.