PLA2 promotes fusion between PMN‐specific granules and complex liposomes
dc.contributor.author | Blackwood, R. Alexander | |
dc.contributor.author | Transue, Amy T. | |
dc.contributor.author | Harsh, Donna M. | |
dc.contributor.author | Brower, Ruth C. | |
dc.contributor.author | Zacharek, Sima J. | |
dc.contributor.author | Smolen, James E. | |
dc.contributor.author | Hessler, Ronald J. | |
dc.date.accessioned | 2018-02-05T16:37:58Z | |
dc.date.available | 2018-02-05T16:37:58Z | |
dc.date.issued | 1996-05 | |
dc.identifier.citation | Blackwood, R. Alexander; Transue, Amy T.; Harsh, Donna M.; Brower, Ruth C.; Zacharek, Sima J.; Smolen, James E.; Hessler, Ronald J. (1996). "PLA2 promotes fusion between PMN‐specific granules and complex liposomes." Journal of Leukocyte Biology 59(5): 663-670. | |
dc.identifier.issn | 0741-5400 | |
dc.identifier.issn | 1938-3673 | |
dc.identifier.uri | https://hdl.handle.net/2027.42/141645 | |
dc.description.abstract | Neutrophil stimulation results in the activation of a variety of phospholipases, including phospholipase A2 (PLA2), which releases arachidonic acid from the 2 position of membrane phospholipids, leaving a lysophospholipid. Because arachidonic acid is known to be a potent fusogen in vitro, we examined the effect of metabolism by PLA2 on the fusion of complex liposomes (liposomes prepared with a phospholipid composition similar to that found in neutrophil plasma membrane). We observed that PLA2 augmented the fusion of complex liposomes with each other as well as with specific granules isolated from human neutrophils, lowering the Ca2+ requirement for fusion by three orders of magnitude. Furthermore, although lysophospholipids inhibited fusion, the incorporation of arachidonic acid into liposome membranes overcame the inhibitory effects of the lysophospholipids. Thus with PLA2 and annexins we were able to obtain fusion of complex liposomes at concentations of Ca2+ that are close to physiological. Our data suggest that the activation of PLA2 and the generation of arachidonic acid may be the major fusion‐promoting event mediating neutrophil degranulation. | |
dc.publisher | Wiley Periodicals, Inc. | |
dc.subject.other | neutrophils | |
dc.subject.other | arachidonic acid | |
dc.subject.other | degranulation | |
dc.subject.other | phosphatidylcholine | |
dc.title | PLA2 promotes fusion between PMN‐specific granules and complex liposomes | |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | |
dc.subject.hlbsecondlevel | Microbiology and Immunology | |
dc.subject.hlbtoplevel | Health Sciences | |
dc.description.peerreviewed | Peer Reviewed | |
dc.contributor.affiliationum | Department of Pediatries, Division of Infectious Diseases and Hematology‐Oncology, University of Michigan Medical Center, C.S. Mott Children’s Hospital, Ann Arbor | |
dc.description.bitstreamurl | https://deepblue.lib.umich.edu/bitstream/2027.42/141645/1/jlb0663.pdf | |
dc.identifier.doi | 10.1002/jlb.59.5.663 | |
dc.identifier.source | Journal of Leukocyte Biology | |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.