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Optimizing Multiplexed Detections of Diabetes Antibodies via Quantitative Microfluidic Droplet Array
dc.contributor.authorDuan, Kai
dc.contributor.authorGhosh, Gargi
dc.contributor.authorLo, Joe Fujiou
dc.date.accessioned2018-02-05T16:43:57Z
dc.date.available2019-01-07T18:34:38Zen
dc.date.issued2017-12
dc.identifier.citationDuan, Kai; Ghosh, Gargi; Lo, Joe Fujiou (2017). "Optimizing Multiplexed Detections of Diabetes Antibodies via Quantitative Microfluidic Droplet Array." Small 13(46): n/a-n/a.
dc.identifier.issn1613-6810
dc.identifier.issn1613-6829
dc.identifier.urihttps://hdl.handle.net/2027.42/141961
dc.description.abstractSensitive, single volume detections of multiple diabetes antibodies can provide immunoprofiling and early screening of at‐risk patients. To advance the state‐of‐the‐art suspension assays for diabetes antibodies, porous hydrogel droplets are leveraged in microfluidic serpentine arrays to enhance reagent transport. This spatially multiplexed assay is applied to the detection of antibodies against insulin, glutamic acid decarboxylase, and insulinoma‐associated protein 2. Optimization of assay protocol results in a shortened assay time of 2 h, with better than 20 pg mL Supporting Information detection limits across all three antibodies. Specificity and cross‐reactivity tests show negligible background, nonspecific antibody–antigen, and nonspecific antibody–antibody bindings. Multiplexed detections are able to measure within 15% of target concentrations from low to high ranges. The technique enables quantifications of as little as 8000 molecules in each 500 µm droplet in a single volume, multiplexed assay format, a breakthrough necessary for the adoption of diabetes panels for clinical screening and monitoring in the future.To advance state‐of‐the‐art suspension assays for diabetes antibodies, porous hydrogel droplets are leveraged in microfluidic serpentine arrays to enhance the detection of antibodies against insulin, glutamic acid decarboxylase, and insulinoma associated protein‐2. The technique enables sensitive detection in a single volume, multiplexed format to facilitate assay adoption for clinical screening and monitoring in the future.
dc.publisherMayo Medical Laboratories
dc.publisherWiley Periodicals, Inc.
dc.subject.otherantibody
dc.subject.othermultiplexing
dc.subject.othermicrofluidic
dc.subject.otherdroplets
dc.subject.otherdiabetes
dc.titleOptimizing Multiplexed Detections of Diabetes Antibodies via Quantitative Microfluidic Droplet Array
dc.typeArticleen_US
dc.rights.robotsIndexNoFollow
dc.subject.hlbsecondlevelPhysics
dc.subject.hlbsecondlevelMaterials Science and Engineering
dc.subject.hlbtoplevelEngineering
dc.subject.hlbtoplevelScience
dc.description.peerreviewedPeer Reviewed
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/141961/1/smll201702323-sup-0001-S1.pdf
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/141961/2/smll201702323_am.pdf
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/141961/3/smll201702323.pdf
dc.identifier.doi10.1002/smll.201702323
dc.identifier.sourceSmall
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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