Differentiating agents regulate cathepsin B gene expression in HL‐60 cells
dc.contributor.author | Berquin, Isabelle M. | |
dc.contributor.author | Yan, Shiqing | |
dc.contributor.author | Katiyar, Kamna | |
dc.contributor.author | Huang, Li | |
dc.contributor.author | Sloane, Bonnie F. | |
dc.contributor.author | Troen, Bruce R. | |
dc.date.accessioned | 2018-02-05T16:49:06Z | |
dc.date.available | 2018-02-05T16:49:06Z | |
dc.date.issued | 1999-10 | |
dc.identifier.citation | Berquin, Isabelle M.; Yan, Shiqing; Katiyar, Kamna; Huang, Li; Sloane, Bonnie F.; Troen, Bruce R. (1999). "Differentiating agents regulate cathepsin B gene expression in HL‐60 cells." Journal of Leukocyte Biology 66(4): 609-616. | |
dc.identifier.issn | 0741-5400 | |
dc.identifier.issn | 1938-3673 | |
dc.identifier.uri | https://hdl.handle.net/2027.42/142263 | |
dc.description.abstract | We utilized HL‐60 cells as a model system to examine the regulation of ctsb gene expression by differentiating agents. Inducers of monocytic differentiation [phorbol ester (PMA), calcitriol (D3), and sodium butyrate (NaB)] and inducers of granulocytic differentiation [all‐trans retinoic acid (RA) and 9‐cis retinoic acid (9‐cis RA)] increase ctsb mRNA levels in a dose‐dependent manner as determined by Northern blot hybridization. D3 and retinoids exert additive effects, suggesting that these agents act in part through distinct pathways. Actinomycin D decay experiments indicate that D3, NaB, RA, and 9‐cis RA do not alter mRNA stability. In contrast, PMA markedly increases the half‐life of ctsb mRNA. In transient transfection assays, PMA and NaB both stimulate transcription of the luciferase reporter gene placed under the control of ctsb promoter fragments. Thus, inducers of HL‐60 cell differentiation can regulate the expression of the ctsb gene at both transcriptional and posttranscriptional levels. J. Leukoc. Biol. 66: 609–616; 1999. | |
dc.publisher | Wiley Periodicals, Inc. | |
dc.subject.other | mRNA | |
dc.subject.other | posttranscriptional regulation | |
dc.subject.other | monocyte | |
dc.subject.other | granulocyte | |
dc.subject.other | transcriptional regulation | |
dc.title | Differentiating agents regulate cathepsin B gene expression in HL‐60 cells | |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | |
dc.subject.hlbsecondlevel | Microbiology and Immunology | |
dc.subject.hlbtoplevel | Health Sciences | |
dc.description.peerreviewed | Peer Reviewed | |
dc.contributor.affiliationum | Department of Internal Medicine, Division of Geriatric Medicine, Institute of Gerontology, Geriatric Research Education and Clinical Center, Veterans Administration Medical Center, University of Michigan, Ann Arbor | |
dc.contributor.affiliationother | Lankenau Medical Research Center Thomas Jefferson University, Wynnewood, Pennsylvania | |
dc.contributor.affiliationother | Department of Pharmacology, Wayne State University School of Medicine, Detroit, Michigan | |
dc.description.bitstreamurl | https://deepblue.lib.umich.edu/bitstream/2027.42/142263/1/jlb0609.pdf | |
dc.identifier.doi | 10.1002/jlb.66.4.609 | |
dc.identifier.source | Journal of Leukocyte Biology | |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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