Formation of pH‐Resistant Monodispersed Polymer–Lipid Nanodiscs
dc.contributor.author | Ravula, Thirupathi | |
dc.contributor.author | Hardin, Nathaniel Z. | |
dc.contributor.author | Ramadugu, Sudheer Kumar | |
dc.contributor.author | Cox, Sarah J. | |
dc.contributor.author | Ramamoorthy, Ayyalusamy | |
dc.date.accessioned | 2018-02-05T16:49:15Z | |
dc.date.available | 2019-03-01T21:00:18Z | en |
dc.date.issued | 2018-01-26 | |
dc.identifier.citation | Ravula, Thirupathi; Hardin, Nathaniel Z.; Ramadugu, Sudheer Kumar; Cox, Sarah J.; Ramamoorthy, Ayyalusamy (2018). "Formation of pH‐Resistant Monodispersed Polymer–Lipid Nanodiscs." Angewandte Chemie 130(5): 1356-1359. | |
dc.identifier.issn | 0044-8249 | |
dc.identifier.issn | 1521-3757 | |
dc.identifier.uri | https://hdl.handle.net/2027.42/142270 | |
dc.description.abstract | Polymer lipid nanodiscs are an invaluable system for structural and functional studies of membrane proteins in their near‐native environment. Despite the recent advances in the development and usage of polymer lipid nanodisc systems, lack of control over size and poor tolerance to pH and divalent metal ions are major limitations for further applications. A facile modification of a low‐molecular‐weight styrene maleic acid copolymer is demonstrated to form monodispersed lipid bilayer nanodiscs that show ultra‐stability towards divalent metal ion concentration over a pH range of 2.5 to 10. The macro‐nanodiscs (>20 nm diameter) show magnetic alignment properties that can be exploited for high‐resolution structural studies of membrane proteins and amyloid proteins using solid‐state NMR techniques. The new polymer, SMA‐QA, nanodisc is a robust membrane mimetic tool that offers significant advantages over currently reported nanodisc systems.Lösung für viele Probleme: Polymernanoscheiben sind üblicherweise nur schlecht mit pH‐Änderungen und zweifach geladenen Metallionen verträglich, außerdem lässt sich ihre Größe nur schwer einstellen. Ein modifiziertes Styrol‐Maleimid‐Polymer bildet hingegen monodisperse Nanoscheiben, die zwischen pH 2.5 und 10 gegenüber zweifach geladenen Metallionen stabil sind. | |
dc.publisher | Wiley Periodicals, Inc. | |
dc.subject.other | Lipiddoppelschichten | |
dc.subject.other | pH-Verträglichkeit | |
dc.subject.other | Polymernanoscheiben | |
dc.subject.other | Styrol-Maleimid-Polymere | |
dc.subject.other | Magnetische Ausrichtung | |
dc.title | Formation of pH‐Resistant Monodispersed Polymer–Lipid Nanodiscs | |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | |
dc.subject.hlbsecondlevel | Materials Science and Engineering | |
dc.subject.hlbsecondlevel | Chemical Engineering | |
dc.subject.hlbsecondlevel | Chemistry | |
dc.subject.hlbtoplevel | Engineering | |
dc.subject.hlbtoplevel | Science | |
dc.description.peerreviewed | Peer Reviewed | |
dc.description.bitstreamurl | https://deepblue.lib.umich.edu/bitstream/2027.42/142270/1/ange201712017_am.pdf | |
dc.description.bitstreamurl | https://deepblue.lib.umich.edu/bitstream/2027.42/142270/2/ange201712017-sup-0001-misc_information.pdf | |
dc.description.bitstreamurl | https://deepblue.lib.umich.edu/bitstream/2027.42/142270/3/ange201712017.pdf | |
dc.identifier.doi | 10.1002/ange.201712017 | |
dc.identifier.source | Angewandte Chemie | |
dc.identifier.citedreference | E. S. Salnikov, C. Aisenbrey, F. Aussenac, O. Ouari, H. Sarrouj, C. Reiter, P. Tordo, F. Engelke, B. Bechinger, Sci. Rep. 2016, 6, 20895. | |
dc.identifier.citedreference | F. Hagn, G. Wagner, J. Biomol. NMR 2015, 61, 249 – 260. | |
dc.identifier.citedreference | I. G. Denisov, S. G. Sligar, Chem. Rev. 2017, 117, 4669 – 4713. | |
dc.identifier.citedreference | S. C. Lee, N. L. Pollock, Biochem. Soc. Trans. 2016, 44, 1011 – 1018. | |
dc.identifier.citedreference | T. H. Bayburt, Y. V. Grinkova, S. G. Sligar, Nano Lett. 2002, 2, 853 – 856. | |
dc.identifier.citedreference | M. L. Nasr, D. Baptista, M. Strauss, Z. J. Sun, S. Grigoriu, S. Huser, A. Pluckthun, F. Hagn, T. Walz, J. M. Hogle, G. Wagner, Nat. Methods 2017, 14, 49 – 52. | |
dc.identifier.citedreference | T. H. Bayburt, S. G. Sligar, FEBS Lett. 2010, 584, 1721 – 1727. | |
dc.identifier.citedreference | M. Zhang, R. Huang, R. Ackermann, S. C. Im, L. Waskell, A. Schwendeman, A. Ramamoorthy, Angew. Chem. Int. Ed. 2016, 55, 4497 – 4499; Angew. Chem. 2016, 128, 4573 – 4575. | |
dc.identifier.citedreference | A. Oluwole, B. Danielczak, A. Meister, J. Babalola, C. Vargas, S. Keller, Angew. Chem. Int. Ed. 2017, 56, 1919 – 1924; Angew. Chem. 2017, 129, 1946 – 1951. | |
dc.identifier.citedreference | M. C. Orwick, P. J. Judge, J. Procek, L. Lindholm, A. Graziadei, A. Engel, G. Gröbner, A. Watts, Angew. Chem. Int. Ed. 2012, 51, 4653 – 4657; Angew. Chem. 2012, 124, 4731 – 4735. | |
dc.identifier.citedreference | T. Ravula, S. K. Ramadugu, G. Di Mauro, A. Ramamoorthy, Angew. Chem. Int. Ed. 2017, 56, 11466 – 11470; Angew. Chem. 2017, 129, 11624 – 11628. | |
dc.identifier.citedreference | I. D. Sahu, R. Zhang, M. M. Dunagan, A. F. Craig, G. A. Lorigan, J. Phys. Chem. B 2017, 121, 5312 – 5321. | |
dc.identifier.citedreference | S. C. Lee, T. J. Knowles, V. L. G. Postis, M. Jamshad, R. A. Parslow, Y.-p. Lin, A. Goldman, P. Sridhar, M. Overduin, S. P. Muench, T. R. Dafforn, Nat. Protoc. 2016, 11, 1149 – 1162. | |
dc.identifier.citedreference | J. M. Dörr, M. C. Koorengevel, M. Schafer, A. V. Prokofyev, S. Scheidelaar, E. A. van der Cruijsen, T. R. Dafforn, M. Baldus, J. A. Killian, Proc. Natl. Acad. Sci. USA 2014, 111, 18607 – 18612. | |
dc.identifier.citedreference | V. S. K. Ramadugu, G. M. Di Mauro, T. Ravula, A. Ramamoorthy, Chem. Commun. 2017, 53, 10824 – 10826. | |
dc.identifier.citedreference | S. V. Dvinskikh, K. Yamamoto, U. H. Durr, A. Ramamoorthy, J. Magn. Reson. 2007, 184, 228 – 235. | |
dc.identifier.citedreference | T. Ravula, N. Z. Hardin, S. K. Ramadugu, A. Ramamoorthy, Langmuir 2017, 33, 10655 – 10662. | |
dc.identifier.citedreference | S. H. Park, S. Berkamp, G. A. Cook, M. K. Chan, H. Viadiu, S. J. Opella, Biochemistry 2011, 50, 8983 – 8985. | |
dc.identifier.citedreference | U. H. Dürr, M. Gildenberg, A. Ramamoorthy, Chem. Rev. 2012, 112, 6054 – 6074. | |
dc.identifier.citedreference | H. Qin, Y. Miao, T. A. Cross, R. Fu, J. Phys. Chem. B 2017, 121, 4799 – 4809. | |
dc.identifier.citedreference | F. Hagn, M. Etzkorn, T. Raschle, G. Wagner, J. Am. Chem. Soc. 2013, 135, 1919 – 1925. | |
dc.identifier.citedreference | V. Postis, S. Rawson, J. K. Mitchell, S. C. Lee, R. A. Parslow, T. R. Dafforn, S. A. Baldwin, S. P. Muench, Biochim. Biophys. Acta Biomembr. 2015, 1848, 496 – 501. | |
dc.identifier.citedreference | L. S. Brown, V. Ladizhansky, Protein Sci. 2015, 24, 1333 – 1346. | |
dc.identifier.citedreference | D. P. Staus, R. T. Strachan, A. Manglik, B. Pani, A. W. Kahsai, T. H. Kim, L. M. Wingler, S. Ahn, A. Chatterjee, A. Masoudi, A. C. Kruse, E. Pardon, J. Steyaert, W. I. Weis, R. S. Prosser, B. K. Kobilka, T. Costa, R. J. Lefkowitz, Nature 2016, 535, 448 – 452. | |
dc.identifier.citedreference | Y. Gao, E. Cao, D. Julius, Y. Cheng, Nature 2016, 534, 347 – 351. | |
dc.identifier.citedreference | N. Das, D. T. Murray, T. A. Cross, Nat. Protoc. 2013, 8, 2256 – 2270. | |
dc.identifier.citedreference | D. C. Rodriguez Camargo, K. J. Korshavn, A. Jussupow, K. Raltchev, D. Goricanec, M. Fleisch, R. Sarkar, K. Xue, M. Aichler, G. Mettenleiter, A. K. Walch, C. Camilloni, F. Hagn, B. Reif, A. Ramamoorthy, eLife 2017, 6, e 31226. | |
dc.identifier.citedreference | R. Ahmed, B. VanSchouwen, N. Jafari, X. Ni, J. Ortega, G. Melacini, J. Am. Chem. Soc. 2017, 139, 13720 – 13734. | |
dc.identifier.citedreference | G. Whitesides, J. Mathias, C. Seto, Science 1991, 254, 1312 – 1319. | |
dc.identifier.citedreference | M. Sarikaya, C. Tamerler, A. K. Y. Jen, K. Schulten, F. Baneyx, Nat. Mater. 2003, 2, 577 – 585. | |
dc.identifier.citedreference | I. G. Denisov, S. G. Sligar, Nat. Struct. Mol. Biol. 2016, 23, 481 – 486. | |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.