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Synthesis of Oligodeoxyribo‐ and Oligoribonucleotides According to the H‐Phosphonate Method

dc.contributor.authorStrömberg, Roger
dc.contributor.authorStawinski, Jacek
dc.date.accessioned2018-05-15T20:12:23Z
dc.date.available2018-05-15T20:12:23Z
dc.date.issued2004-12
dc.identifier.citationStrömberg, Roger ; Stawinski, Jacek (2004). "Synthesis of Oligodeoxyribo‐ and Oligoribonucleotides According to the H‐Phosphonate Method." Current Protocols in Nucleic Acid Chemistry 19(1): 3.4.1-3.4.15.
dc.identifier.issn1934-9270
dc.identifier.issn1934-9289
dc.identifier.urihttps://hdl.handle.net/2027.42/143594
dc.description.abstractOligonucleotides can be synthesized by condensing a protected nucleoside H‐phosphonate monoester with a second nucleoside in the presence of a coupling agent to produce a dinucleoside H‐phosphonate diester. This can then be converted to a dinucleoside phosphate or to a backbone‐modified analog such as a phosphorothioate or phosphoramidite. This unit discusses four alternative methods for synthesizing nucleoside H‐phosphonate monoesters. The methods are efficient and experimentally simple, and use readily available reagents. The unit describes the activation of the monoesters, as well as competing acylation and other potential side reactions.
dc.publisherWiley Periodicals, Inc.
dc.publisherElsevier/North‐Holland
dc.titleSynthesis of Oligodeoxyribo‐ and Oligoribonucleotides According to the H‐Phosphonate Method
dc.typeArticleen_US
dc.rights.robotsIndexNoFollow
dc.subject.hlbsecondlevelBiological Chemistry
dc.subject.hlbsecondlevelChemical Engineering
dc.subject.hlbsecondlevelChemistry
dc.subject.hlbsecondlevelPublic Health
dc.subject.hlbtoplevelHealth Sciences
dc.subject.hlbtoplevelScience
dc.subject.hlbtoplevelEngineering
dc.description.peerreviewedPeer Reviewed
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/143594/1/cpnc0304.pdf
dc.identifier.doi10.1002/0471142700.nc0304s19
dc.identifier.sourceCurrent Protocols in Nucleic Acid Chemistry
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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