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Probing RNA Structures with Hydroxyl Radicals
dc.contributor.authorCelander, Daniel W.
dc.date.accessioned2018-05-15T20:12:39Z
dc.date.available2018-05-15T20:12:39Z
dc.date.issued2000-02
dc.identifier.citationCelander, Daniel W. (2000). "Probing RNA Structures with Hydroxyl Radicals." Current Protocols in Nucleic Acid Chemistry 00(1): 6.5.1-6.5.6.
dc.identifier.issn1934-9270
dc.identifier.issn1934-9289
dc.identifier.urihttps://hdl.handle.net/2027.42/143607
dc.description.abstractFe(II)‐EDTA can be used to conveniently generate hydroxyl radicals to promote cleavage of RNA at nucleotide resolution. Two procedures are described, involving the generation of free radicals from solvated molecular oxygen and from hydrogen peroxide added to the RNA solution. Unlike other footprinting reagents, hydroxyl radicals cleave the sugar‐phosphate backbone at every residue and thus provide uniform cleavage in a given RNA secondary structure. Because some positions become protected by tertiary folding, this reagent is useful for monitoring the global folding of RNA at equilibrium.
dc.publisherWiley Periodicals, Inc.
dc.titleProbing RNA Structures with Hydroxyl Radicals
dc.typeArticleen_US
dc.rights.robotsIndexNoFollow
dc.subject.hlbsecondlevelChemistry
dc.subject.hlbsecondlevelBiological Chemistry
dc.subject.hlbsecondlevelChemical Engineering
dc.subject.hlbsecondlevelPublic Health
dc.subject.hlbtoplevelHealth Sciences
dc.subject.hlbtoplevelScience
dc.subject.hlbtoplevelEngineering
dc.description.peerreviewedPeer Reviewed
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/143607/1/cpnc0605.pdf
dc.identifier.doi10.1002/0471142700.nc0605s00
dc.identifier.sourceCurrent Protocols in Nucleic Acid Chemistry
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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