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Engineering Specific Cross‐Links in Nucleic Acids Using Glycol Linkers
dc.contributor.authorO’Dea, Timothy
dc.contributor.authorMcLaughlin, Larry W.
dc.date.accessioned2018-05-15T20:12:57Z
dc.date.available2018-05-15T20:12:57Z
dc.date.issued2000-02
dc.identifier.citationO’Dea, Timothy; McLaughlin, Larry W. (2000). "Engineering Specific Cross‐Links in Nucleic Acids Using Glycol Linkers." Current Protocols in Nucleic Acid Chemistry 00(1): 5.3.1-5.3.8.
dc.identifier.issn1934-9270
dc.identifier.issn1934-9289
dc.identifier.urihttps://hdl.handle.net/2027.42/143621
dc.description.abstractOne of the most convenient methods for generating oligonucleotides possessing intra‐ or interstrand cross‐links is through incorporation of oligoethylene glycol bridges by solid‐phase synthesis. The reagents are commercially available or can be synthesized in a few easy synthetic steps. Unlike many other DNA and RNA cross‐links, aspects of the structural and thermodynamic impact of modifying nucleic acids with oligoethylene glycols have been studied. This unit covers protection, phosphitylation, and preparation of the glycol linker for oligonucleotide synthesis.
dc.publisherWiley Periodicals, Inc.
dc.titleEngineering Specific Cross‐Links in Nucleic Acids Using Glycol Linkers
dc.typeArticleen_US
dc.rights.robotsIndexNoFollow
dc.subject.hlbsecondlevelChemical Engineering
dc.subject.hlbsecondlevelChemistry
dc.subject.hlbsecondlevelPublic Health
dc.subject.hlbsecondlevelBiological Chemistry
dc.subject.hlbtoplevelHealth Sciences
dc.subject.hlbtoplevelScience
dc.subject.hlbtoplevelEngineering
dc.description.peerreviewedPeer Reviewed
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/143621/1/cpnc0503.pdf
dc.identifier.doi10.1002/0471142700.nc0503s00
dc.identifier.sourceCurrent Protocols in Nucleic Acid Chemistry
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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