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Design, Synthesis, and Amplification of DNA Pools for In Vitro Selection
dc.contributor.authorPollard, Jack
dc.contributor.authorBell, Sabine D.
dc.contributor.authorEllington, Andrew D.
dc.date.accessioned2018-05-15T20:13:00Z
dc.date.available2018-05-15T20:13:00Z
dc.date.issued2000-02
dc.identifier.citationPollard, Jack; Bell, Sabine D.; Ellington, Andrew D. (2000). "Design, Synthesis, and Amplification of DNA Pools for In Vitro Selection." Current Protocols in Nucleic Acid Chemistry 00(1): 9.2.1-9.2.23.
dc.identifier.issn1934-9270
dc.identifier.issn1934-9289
dc.identifier.urihttps://hdl.handle.net/2027.42/143624
dc.description.abstractPreparation of a random‐sequence DNA pool is presented. The degree of randomization and the length of the random sequence are discussed, as is synthesis of the pool using a DNA synthesizer. Purification of a single‐stranded pool and conversion to a double‐stranded pool are presented as step‐by‐step protocols. Support protocols describe determination of the complexity and skewing of the pool, and optimization of amplification conditions.
dc.publisherPerSeptive Biosystems
dc.publisherWiley Periodicals, Inc.
dc.titleDesign, Synthesis, and Amplification of DNA Pools for In Vitro Selection
dc.typeArticleen_US
dc.rights.robotsIndexNoFollow
dc.subject.hlbsecondlevelChemical Engineering
dc.subject.hlbsecondlevelChemistry
dc.subject.hlbsecondlevelPublic Health
dc.subject.hlbsecondlevelBiological Chemistry
dc.subject.hlbtoplevelHealth Sciences
dc.subject.hlbtoplevelScience
dc.subject.hlbtoplevelEngineering
dc.description.peerreviewedPeer Reviewed
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/143624/1/cpnc0902.pdf
dc.identifier.doi10.1002/0471142700.nc0902s00
dc.identifier.sourceCurrent Protocols in Nucleic Acid Chemistry
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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