Design, Synthesis, and Amplification of DNA Pools for In Vitro Selection
dc.contributor.author | Pollard, Jack | |
dc.contributor.author | Bell, Sabine D. | |
dc.contributor.author | Ellington, Andrew D. | |
dc.date.accessioned | 2018-05-15T20:13:00Z | |
dc.date.available | 2018-05-15T20:13:00Z | |
dc.date.issued | 2000-02 | |
dc.identifier.citation | Pollard, Jack; Bell, Sabine D.; Ellington, Andrew D. (2000). "Design, Synthesis, and Amplification of DNA Pools for In Vitro Selection." Current Protocols in Nucleic Acid Chemistry 00(1): 9.2.1-9.2.23. | |
dc.identifier.issn | 1934-9270 | |
dc.identifier.issn | 1934-9289 | |
dc.identifier.uri | https://hdl.handle.net/2027.42/143624 | |
dc.description.abstract | Preparation of a random‐sequence DNA pool is presented. The degree of randomization and the length of the random sequence are discussed, as is synthesis of the pool using a DNA synthesizer. Purification of a single‐stranded pool and conversion to a double‐stranded pool are presented as step‐by‐step protocols. Support protocols describe determination of the complexity and skewing of the pool, and optimization of amplification conditions. | |
dc.publisher | PerSeptive Biosystems | |
dc.publisher | Wiley Periodicals, Inc. | |
dc.title | Design, Synthesis, and Amplification of DNA Pools for In Vitro Selection | |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | |
dc.subject.hlbsecondlevel | Chemical Engineering | |
dc.subject.hlbsecondlevel | Chemistry | |
dc.subject.hlbsecondlevel | Public Health | |
dc.subject.hlbsecondlevel | Biological Chemistry | |
dc.subject.hlbtoplevel | Health Sciences | |
dc.subject.hlbtoplevel | Science | |
dc.subject.hlbtoplevel | Engineering | |
dc.description.peerreviewed | Peer Reviewed | |
dc.description.bitstreamurl | https://deepblue.lib.umich.edu/bitstream/2027.42/143624/1/cpnc0902.pdf | |
dc.identifier.doi | 10.1002/0471142700.nc0902s00 | |
dc.identifier.source | Current Protocols in Nucleic Acid Chemistry | |
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dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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