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Analysis of Oligonucleotides by Matrixâ Assisted Laser Desorption/Ionization Timeâ ofâ Flight Mass Spectrometry
dc.contributor.authorChou, Chau‐wen
dc.contributor.authorLimbach, Patrick A.
dc.date.accessioned2018-05-15T20:13:07Z
dc.date.available2018-05-15T20:13:07Z
dc.date.issued2000-02
dc.identifier.citationChou, Chau‐wen ; Limbach, Patrick A. (2000). "Analysis of Oligonucleotides by Matrixâ Assisted Laser Desorption/Ionization Timeâ ofâ Flight Mass Spectrometry." Current Protocols in Nucleic Acid Chemistry 00(1): 10.1.1-10.1.25.
dc.identifier.issn1934-9270
dc.identifier.issn1934-9289
dc.identifier.urihttps://hdl.handle.net/2027.42/143629
dc.description.abstractMALDIâ MS is one of the most useful techniques available for determining biomolecule mass. It offers high mass accuracy, good sensitivity, simplicity, and speed. Because singly charged ions of oligonucleotides are typically observed, MALDIâ MS spectra are easy to interpret. This unit presents protocols for sample preparation and purification, matrix preparation, and matrix/analyte sample preparation. It provides an introduction to the instrumentation and its calibration, and a discussion of some of the useful applications of MALDIâ MS analysis in the study of oligonucleotides. This technique is typically used for 120â mer or smaller oligonucleotides.
dc.publisherWiley Periodicals, Inc.
dc.titleAnalysis of Oligonucleotides by Matrixâ Assisted Laser Desorption/Ionization Timeâ ofâ Flight Mass Spectrometry
dc.typeArticleen_US
dc.rights.robotsIndexNoFollow
dc.subject.hlbsecondlevelBiological Chemistry
dc.subject.hlbsecondlevelChemical Engineering
dc.subject.hlbsecondlevelChemistry
dc.subject.hlbsecondlevelPublic Health
dc.subject.hlbtoplevelScience
dc.subject.hlbtoplevelEngineering
dc.subject.hlbtoplevelHealth Sciences
dc.description.peerreviewedPeer Reviewed
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/143629/1/cpnc1001.pdf
dc.identifier.doi10.1002/0471142700.nc1001s00
dc.identifier.sourceCurrent Protocols in Nucleic Acid Chemistry
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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