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Synthetic Strategies and Parameters Involved in the Synthesis of Oligodeoxyribonucleotides According to the Phosphoramidite Method
dc.contributor.authorBeaucage, Serge L.
dc.contributor.authorCaruthers, Marvin H.
dc.date.accessioned2018-05-15T20:13:12Z
dc.date.available2018-05-15T20:13:12Z
dc.date.issued2000-02
dc.identifier.citationBeaucage, Serge L.; Caruthers, Marvin H. (2000). "Synthetic Strategies and Parameters Involved in the Synthesis of Oligodeoxyribonucleotides According to the Phosphoramidite Method." Current Protocols in Nucleic Acid Chemistry 00(1): 3.3.1-3.3.20.
dc.identifier.issn1934-9270
dc.identifier.issn1934-9289
dc.identifier.urihttps://hdl.handle.net/2027.42/143633
dc.description.abstractThe phosphoramidite approach has had a major impact on the synthesis of oligonucleotides. This unit describes parameters that affect the performance of this method for preparing oligodeoxyribonucleotides, as well as a number of compatible strategies. Milestones that led to the discovery of the approach are chronologically reported. Alternate strategies are also described to underscore the versatility by which these synthons can be obtained. Mechanisms of deoxyribonucleoside phosphoramidite activation, factors affecting condensation, and deprotection strategies are discussed.
dc.publisherWiley Periodicals, Inc.
dc.publisherPlenum
dc.titleSynthetic Strategies and Parameters Involved in the Synthesis of Oligodeoxyribonucleotides According to the Phosphoramidite Method
dc.typeArticleen_US
dc.rights.robotsIndexNoFollow
dc.subject.hlbsecondlevelChemical Engineering
dc.subject.hlbsecondlevelChemistry
dc.subject.hlbsecondlevelPublic Health
dc.subject.hlbsecondlevelBiological Chemistry
dc.subject.hlbtoplevelEngineering
dc.subject.hlbtoplevelHealth Sciences
dc.subject.hlbtoplevelScience
dc.description.peerreviewedPeer Reviewed
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/143633/1/cpnc0303.pdf
dc.identifier.doi10.1002/0471142700.nc0303s00
dc.identifier.sourceCurrent Protocols in Nucleic Acid Chemistry
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