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Solid‐Phase Synthesis of Branched Oligonucleotides
dc.contributor.authorCarriero, Sandra
dc.contributor.authorDamha, Masad J.
dc.date.accessioned2018-05-15T20:13:13Z
dc.date.available2018-05-15T20:13:13Z
dc.date.issued2002-06
dc.identifier.citationCarriero, Sandra; Damha, Masad J. (2002). "Solid‐Phase Synthesis of Branched Oligonucleotides." Current Protocols in Nucleic Acid Chemistry 9(1): 4.14.1-4.14.32.
dc.identifier.issn1934-9270
dc.identifier.issn1934-9289
dc.identifier.urihttps://hdl.handle.net/2027.42/143634
dc.description.abstractBranched nucleic acids (bNAs) have been of particular interest since the discovery of RNA forks and lariats as intermediates of nuclear mRNA splicing, as well as multicopy, single‐stranded DNA (msDNA). Such molecules contain the inherent trait of vicinal 2′,5′‐ and 3′,5′‐phosphodiester linkages. bNAs have many potential applications in nucleic acid biochemistry, particularly as tools for studying the substrate specificity of lariat debranching enzymes, and as biological probes for the investigation of branch recognition during pre‐mRNA splicing. The protocols described herein allow for the facile solid‐phase synthesis of branched DNA and/or RNA oligonucleotides of varying chain length, containing symmetrical or asymmetrical sequences immediate to an RNA branch point. The synthetic methodology utilizes widely adopted phosphoramidite chemistry. Methods for efficient purification of bNAs via anion‐exchange HPLC and PAGE are also illustrated.
dc.publisherWiley Periodicals, Inc.
dc.publisherAcademic Press
dc.titleSolid‐Phase Synthesis of Branched Oligonucleotides
dc.typeArticleen_US
dc.rights.robotsIndexNoFollow
dc.subject.hlbsecondlevelPublic Health
dc.subject.hlbsecondlevelBiological Chemistry
dc.subject.hlbsecondlevelChemical Engineering
dc.subject.hlbsecondlevelChemistry
dc.subject.hlbtoplevelHealth Sciences
dc.subject.hlbtoplevelScience
dc.subject.hlbtoplevelEngineering
dc.description.peerreviewedPeer Reviewed
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/143634/1/cpnc0414.pdf
dc.identifier.doi10.1002/0471142700.nc0414s09
dc.identifier.sourceCurrent Protocols in Nucleic Acid Chemistry
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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