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Characterization of Tertiary Folding of RNA by Circular Dichroism and Urea
dc.contributor.authorSosnick, Tobin R.
dc.date.accessioned2018-05-15T20:14:29Z
dc.date.available2018-05-15T20:14:29Z
dc.date.issued2001-04
dc.identifier.citationSosnick, Tobin R. (2001). "Characterization of Tertiary Folding of RNA by Circular Dichroism and Urea." Current Protocols in Nucleic Acid Chemistry 4(1): 11.5.1-11.5.10.
dc.identifier.issn1934-9270
dc.identifier.issn1934-9289
dc.identifier.urihttps://hdl.handle.net/2027.42/143699
dc.description.abstractCD spectroscopy can be used to monitor RNA tertiary folding transitions that may not be observable by absorbance spectroscopy. With the use of computer‐controlled titrators, data can be acquired rapidly, and accurate thermodynamic properties can be obtained over a wide variety of conditions. Thus, CD spectroscopy provides a useful complement to site‐resolved or chemical modification methods.
dc.publisherWiley Periodicals, Inc.
dc.titleCharacterization of Tertiary Folding of RNA by Circular Dichroism and Urea
dc.typeArticleen_US
dc.rights.robotsIndexNoFollow
dc.subject.hlbsecondlevelChemistry
dc.subject.hlbsecondlevelChemical Engineering
dc.subject.hlbsecondlevelBiological Chemistry
dc.subject.hlbsecondlevelPublic Health
dc.subject.hlbtoplevelEngineering
dc.subject.hlbtoplevelScience
dc.subject.hlbtoplevelHealth Sciences
dc.description.peerreviewedPeer Reviewed
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/143699/1/cpnc1105.pdf
dc.identifier.doi10.1002/0471142700.nc1105s04
dc.identifier.sourceCurrent Protocols in Nucleic Acid Chemistry
dc.identifier.citedreferenceBanerjee, A.R. and Turner, D.H. 1995. The time dependence of chemical modification reveals slow steps in the folding of a group I ribozyme. Biochemistry 34: 6504 ‐ 6512.
dc.identifier.citedreferenceFang, X., Pan, T., and Sosnick, T.R. 1999. A thermodynamic framework and cooperativity in the tertiary folding of a Mg 2+ ‐dependent ribozyme. Biochemistry 38: 16840 ‐ 16846.
dc.identifier.citedreferenceMakhatadze, G.I. and Privalov, P.L. 1992. Protein interactions with urea and guanidinium chloride. A calorimetric study. J. Mol. Biol 226: 491 ‐ 505.
dc.identifier.citedreferenceMyers, J.K., Pace, C.N., and Scholtz, J.M. 1995. Denaturant m values and heat capacity changes: Relation to changes in accessible surface areas of protein unfolding [published erratum appears in Protein. Sci. 1996 May;5(5):981]. Protein Sci. 4: 2138 ‐ 2148.
dc.identifier.citedreferencePan, T. and Sosnick, T.R. 1997. Intermediates and kinetic traps in the folding of a large ribozyme revealed by circular dichroism and UV absorbance spectroscopies and catalytic activity. Nat. Struct. Biol. 4: 931 ‐ 938.
dc.identifier.citedreferenceSclavi, B., Woodson, S., Sullivan, M., Chance, M.R., and Brenowitz, M. 1997. Time‐resolved synchrotron X‐ray “footprinting,” a new approach to the study of nucleic acid structure and function: Application to protein‐DNA interactions and RNA folding. J. Mol. Biol. 266: 144 ‐ 159.
dc.identifier.citedreferenceShelton, V., Sosnick, T.R., and Pan, T. 1999. Applicability of urea in the thermodynamic analysis of secondary and tertiary RNA folding. Biochemistry 38: 16831 ‐ 16839.
dc.identifier.citedreferenceZarrinkar, P.P. and Williamson, J.R. 1994. Kinetic intermediates in RNA folding. Science 265: 918 ‐ 924.
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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