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Attachment of Nucleosides to Solid‐Phase Supports
dc.contributor.authorPon, Richard T.
dc.date.accessioned2018-05-15T20:14:53Z
dc.date.available2018-05-15T20:14:53Z
dc.date.issued2000-02
dc.identifier.citationPon, Richard T. (2000). "Attachment of Nucleosides to Solid‐Phase Supports." Current Protocols in Nucleic Acid Chemistry 00(1): 3.2.1-3.2.23.
dc.identifier.issn1934-9270
dc.identifier.issn1934-9289
dc.identifier.urihttps://hdl.handle.net/2027.42/143722
dc.description.abstractSpecific step‐by‐step instructions are given for coupling nucleosides to LCAA‐CPG supports (supports consisting of a long‐chain alkylamine linked to controlled‐pore glass). Protocols are given for a succinic acid linker and a hydroquinone‐O,O′‐diacetic acid linker. The former is the most widely used linker arm, and the starting materials are widely available. The latter offers greater compatibility with base‐sensitive sequence modifications and great synthetic throughput because it can be cleaved under milder and faster conditions. Additional guidelines are given for selecting a linker arm and coupling protocol. Almost any application requiring synthetic oligonucletodies can be satisfied using one of these linker arms.
dc.publisherESCOM
dc.publisherWiley Periodicals, Inc.
dc.titleAttachment of Nucleosides to Solid‐Phase Supports
dc.typeArticleen_US
dc.rights.robotsIndexNoFollow
dc.subject.hlbsecondlevelChemical Engineering
dc.subject.hlbsecondlevelChemistry
dc.subject.hlbsecondlevelPublic Health
dc.subject.hlbsecondlevelBiological Chemistry
dc.subject.hlbtoplevelHealth Sciences
dc.subject.hlbtoplevelScience
dc.subject.hlbtoplevelEngineering
dc.description.peerreviewedPeer Reviewed
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/143722/1/cpnc0302.pdf
dc.identifier.doi10.1002/0471142700.nc0302s00
dc.identifier.sourceCurrent Protocols in Nucleic Acid Chemistry
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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