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Engineering Disulfide Cross‐Links in RNA Using Thiol‐Disulfide Interchange Chemistry
dc.contributor.authorCohen, Scott B.
dc.contributor.authorCech, Thomas R.
dc.date.accessioned2018-05-15T20:14:56Z
dc.date.available2018-05-15T20:14:56Z
dc.date.issued2000-02
dc.identifier.citationCohen, Scott B.; Cech, Thomas R. (2000). "Engineering Disulfide Cross‐Links in RNA Using Thiol‐Disulfide Interchange Chemistry." Current Protocols in Nucleic Acid Chemistry 00(1): 5.1.1-5.1.10.
dc.identifier.issn1934-9270
dc.identifier.issn1934-9289
dc.identifier.urihttps://hdl.handle.net/2027.42/143724
dc.description.abstractProtocols for postsynthetic modification of 2‐amino‐containing oligoribonucleotides with either an alkyl‐phenyl disulfide or an alkyl thiol group are described. These groups react under mild conditions to form disulfide cross‐links by thiol‐disulfide interchange. These reactants do not form a disulfide bond when incorporated on opposite faces of a short continuous RNA helix, but do form disulfide bonds rapidly when they are placed in proximity. In addition, by incorporating these groups at various positions on large RNAs by semisynthesis, the dynamics of thermal motions can be detected. Such motions are believed to be linked to biological function, and the protocols presented in this unit are among the few simple ways to assess such dynamics.
dc.publisherJohn Wiley & Sons
dc.titleEngineering Disulfide Cross‐Links in RNA Using Thiol‐Disulfide Interchange Chemistry
dc.typeArticleen_US
dc.rights.robotsIndexNoFollow
dc.subject.hlbsecondlevelBiological Chemistry
dc.subject.hlbsecondlevelChemical Engineering
dc.subject.hlbsecondlevelChemistry
dc.subject.hlbsecondlevelPublic Health
dc.subject.hlbtoplevelHealth Sciences
dc.subject.hlbtoplevelScience
dc.subject.hlbtoplevelEngineering
dc.description.peerreviewedPeer Reviewed
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/143724/1/cpnc0501.pdf
dc.identifier.doi10.1002/0471142700.nc0501s00
dc.identifier.sourceCurrent Protocols in Nucleic Acid Chemistry
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dc.identifier.citedreferenceCohen, S.B. and Cech, T.R. 1997. Dynamics of thermal motions within a large catalytic RNA investigated by cross‐linking with thiol‐disulfide interchange. J. Am. Chem. Soc. 119: 6259 ‐ 6268.
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dc.identifier.citedreferenceCohen and Cech, 1997.See above.
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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