Engineering Disulfide Cross‐Links in RNA Using Thiol‐Disulfide Interchange Chemistry
dc.contributor.author | Cohen, Scott B. | |
dc.contributor.author | Cech, Thomas R. | |
dc.date.accessioned | 2018-05-15T20:14:56Z | |
dc.date.available | 2018-05-15T20:14:56Z | |
dc.date.issued | 2000-02 | |
dc.identifier.citation | Cohen, Scott B.; Cech, Thomas R. (2000). "Engineering Disulfide Cross‐Links in RNA Using Thiol‐Disulfide Interchange Chemistry." Current Protocols in Nucleic Acid Chemistry 00(1): 5.1.1-5.1.10. | |
dc.identifier.issn | 1934-9270 | |
dc.identifier.issn | 1934-9289 | |
dc.identifier.uri | https://hdl.handle.net/2027.42/143724 | |
dc.description.abstract | Protocols for postsynthetic modification of 2‐amino‐containing oligoribonucleotides with either an alkyl‐phenyl disulfide or an alkyl thiol group are described. These groups react under mild conditions to form disulfide cross‐links by thiol‐disulfide interchange. These reactants do not form a disulfide bond when incorporated on opposite faces of a short continuous RNA helix, but do form disulfide bonds rapidly when they are placed in proximity. In addition, by incorporating these groups at various positions on large RNAs by semisynthesis, the dynamics of thermal motions can be detected. Such motions are believed to be linked to biological function, and the protocols presented in this unit are among the few simple ways to assess such dynamics. | |
dc.publisher | John Wiley & Sons | |
dc.title | Engineering Disulfide Cross‐Links in RNA Using Thiol‐Disulfide Interchange Chemistry | |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | |
dc.subject.hlbsecondlevel | Biological Chemistry | |
dc.subject.hlbsecondlevel | Chemical Engineering | |
dc.subject.hlbsecondlevel | Chemistry | |
dc.subject.hlbsecondlevel | Public Health | |
dc.subject.hlbtoplevel | Health Sciences | |
dc.subject.hlbtoplevel | Science | |
dc.subject.hlbtoplevel | Engineering | |
dc.description.peerreviewed | Peer Reviewed | |
dc.description.bitstreamurl | https://deepblue.lib.umich.edu/bitstream/2027.42/143724/1/cpnc0501.pdf | |
dc.identifier.doi | 10.1002/0471142700.nc0501s00 | |
dc.identifier.source | Current Protocols in Nucleic Acid Chemistry | |
dc.identifier.citedreference | Wincott, F., DiRenzo, A., Shaffer, C., Grimm, S., Tracz, D., Workman, C., Sweedler, D., Gonzalez, C., Scaringe, S., and Usman, N. 1995. Synthesis, deprotection, analysis. and purification of RNA and ribozymes. Nucl. Acids Res. 23: 2677 ‐ 2684. | |
dc.identifier.citedreference | Sigurdsson et al., 1995.See above. | |
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dc.identifier.citedreference | Chen, C.B. and Sigman, D.S. 1988. Sequence‐ specific scission of RNA by 1,10‐ phenanthroline‐ copper linked to deoxyoligonucleotides. J. Am. Chem. Soc. 110: 6570 ‐ 6572. | |
dc.identifier.citedreference | Cohen, S.B. and Cech, T.R. 1997. Dynamics of thermal motions within a large catalytic RNA investigated by cross‐linking with thiol‐disulfide interchange. J. Am. Chem. Soc. 119: 6259 ‐ 6268. | |
dc.identifier.citedreference | Goodwin, J.T., Osborne, S.E., Scholle, E.J., and Glick, G.D. 1996. Design, synthesis, and analysis of yeast tRNA Phe analogs possessing intra‐ and interhelical disulfide cross‐links. J. Am. Chem. Soc. 118: 5207 ‐ 5215. | |
dc.identifier.citedreference | Imazawa, M. and Eckstein, F. 1979. Facile synthesis of 2′‐amino‐2′‐deoxyribo‐furanosyl purines. J. Org. Chem. 44: 2039 ‐ 2041. | |
dc.identifier.citedreference | Moore, M.J. and Sharp, P.A. 1992. Site‐specific modification of pre‐mRNA: the 2′‐hydroxyl groups at the splice sites. Science 256: 992 ‐ 997. | |
dc.identifier.citedreference | Patai, S. 1974. The Chemistry of the Thiol Group. John Wiley & Sons, New York, N.Y. | |
dc.identifier.citedreference | Sambrook, J., Fritsch, E.F., and Maniatis, T. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y. | |
dc.identifier.citedreference | Sigurdsson, S.T., Tuschl, T., and Eckstein, F. 1995. Probing RNA tertiary structure: Interhelical cross‐linking of the hammerhead ribozyme. RNA 1: 575 ‐ 583. | |
dc.identifier.citedreference | Verheyden, J.P.H., Wagner, D., and Moffatt, J.G. 1971. Synthesis of some pyrimidine 2′‐amino‐2′‐deoxynucleosides. J. Org. Chem. 36: 250 ‐ 254. | |
dc.identifier.citedreference | Cohen and Cech, 1997.See above. | |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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