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Probing RNA Structure with Chemical Reagents and Enzymes
dc.contributor.authorZiehler, William A.
dc.contributor.authorEngelke, David R.
dc.date.accessioned2018-05-15T20:15:05Z
dc.date.available2018-05-15T20:15:05Z
dc.date.issued2000-02
dc.identifier.citationZiehler, William A.; Engelke, David R. (2000). "Probing RNA Structure with Chemical Reagents and Enzymes." Current Protocols in Nucleic Acid Chemistry 00(1): 6.1.1-6.1.21.
dc.identifier.issn1934-9270
dc.identifier.issn1934-9289
dc.identifier.urihttps://hdl.handle.net/2027.42/143733
dc.description.abstractThis unit provides thorough coverage of the most useful chemical and enzyme probes that can be used to examine RNA secondary and tertiary structure. Footprinting methods are presented using dimethyl sulfate, diethyl pyrocarbonate, ethylnitrosourea, kethoxal, CMCT, and nucleases. For chemical probes, both strand scission and primer extension detection protocols are included.
dc.publisherWiley Periodicals, Inc.
dc.titleProbing RNA Structure with Chemical Reagents and Enzymes
dc.typeArticleen_US
dc.rights.robotsIndexNoFollow
dc.subject.hlbsecondlevelPublic Health
dc.subject.hlbsecondlevelBiological Chemistry
dc.subject.hlbsecondlevelChemical Engineering
dc.subject.hlbsecondlevelChemistry
dc.subject.hlbtoplevelHealth Sciences
dc.subject.hlbtoplevelScience
dc.subject.hlbtoplevelEngineering
dc.description.peerreviewedPeer Reviewed
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/143733/1/cpnc0601.pdf
dc.identifier.doi10.1002/0471142700.nc0601s00
dc.identifier.sourceCurrent Protocols in Nucleic Acid Chemistry
dc.identifier.citedreferenceEngland, T., Bruce, A., and Uhlenbeck, O. 1980. Specific labeling of 3′ termini of RNA with T4 RNA ligase. Methods Enzymol. 65: 65 ‐ 74.
dc.identifier.citedreferenceGautheret, D., Major, F., and Cedergren, R. 1990. Computer modeling and display of RNA secondary and tertiary structures. Methods Enzymol. 183: 318 ‐ 330.
dc.identifier.citedreferenceGorodkin, J., Heyer, L., and Stormo, G. 1997. Finding the most significant common sequence and structure motifs in a set of RNA sequences. Nucl. Acids Res. 25: 3724 ‐ 3732.
dc.identifier.citedreferenceKnapp, G. 1989. Enzymatic approaches to probing of RNA secondary and tertiary structure. Methods Enzymol. 180: 192 ‐ 212.
dc.identifier.citedreferenceKrol, A. Carbon, P. 1989. A guide for probing native small nuclear RNA and ribonucleoprotein structures. Methods Enzymol. 180: 212 ‐ 227.
dc.identifier.citedreferenceMajor, F., Turcotte, M., Gautheret, D., Lapalme, G., Fillion, E., and Cedergren, R. 1991. The combination of symbolic and numerical computation for three‐dimensional modeling of RNA. Science 253: 1255 ‐ 1260.
dc.identifier.citedreferenceMalhotra, A., Gabb, H., and Harvey, S. 1993. Modeling large nucleic acids. Curr. Opin. Struct. Biol. 3: 241 ‐ 246.
dc.identifier.citedreferenceMilligan, J.F. Uhlenbeck, O.C. 1989. Synthesis of small RNAs using T7 RNA polymerase. Methods Enzymol. 180: 51 ‐ 62.
dc.identifier.citedreferencePeattie, D.A. Gilbert, W. 1980. Chemical probes for higher‐order structure in RNA. Proc. Natl. Acad. Sci. U.S.A. 77: 4679 ‐ 4682.
dc.identifier.citedreferenceWalter, A.E., Turner, D.H., Kim, J., Lyttle, M.H., Muller, P., Matthews, D.H., and Zuker, M. 1994. Coaxial stacking of helixes enhances binding of oligoribonucleotides and improves predictions of RNA folding. Proc. Natl. Acad. Sci. U.S.A. 91: 9218 ‐ 9222.
dc.identifier.citedreferenceZuker, M. 1989. On finding all suboptimal foldings of an RNA molecule. Science 244: 48 ‐ 52.
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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