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Optical Methods
dc.contributor.authorPlum, G. Eric
dc.date.accessioned2018-05-15T20:15:18Z
dc.date.available2018-05-15T20:15:18Z
dc.date.issued2000-02
dc.identifier.citationPlum, G. Eric (2000). "Optical Methods." Current Protocols in Nucleic Acid Chemistry 00(1): 7.3.1-7.3.17.
dc.identifier.issn1934-9270
dc.identifier.issn1934-9289
dc.identifier.urihttps://hdl.handle.net/2027.42/143743
dc.description.abstractEquilibrium optical melting curves are used in this unit to determine thermodynamic parameters of nucleic acid complex formation. This section contains all of the relevant equations and a discussion of which are most appropriate to a given situation. Additionally, procedures are given for making preliminary determinations of molar extinction coefficients and for determining the number of oligonucleotides in a complex. The section on extinction coefficients is particularly essential to anyone needing to know solution concentrations.
dc.publisherElsevier Science Ltd.
dc.publisherWiley Periodicals, Inc.
dc.titleOptical Methods
dc.typeArticleen_US
dc.rights.robotsIndexNoFollow
dc.subject.hlbsecondlevelPublic Health
dc.subject.hlbsecondlevelBiological Chemistry
dc.subject.hlbsecondlevelChemical Engineering
dc.subject.hlbsecondlevelChemistry
dc.subject.hlbtoplevelHealth Sciences
dc.subject.hlbtoplevelScience
dc.subject.hlbtoplevelEngineering
dc.description.peerreviewedPeer Reviewed
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/143743/1/cpnc0703.pdf
dc.identifier.doi10.1002/0471142700.nc0703s00
dc.identifier.sourceCurrent Protocols in Nucleic Acid Chemistry
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dc.identifier.citedreferencePlum, G.E., Grollman, A.P., Johnson, F., and Breslauer, K.J. 1995a. Influence of the oxidatively damaged adduct 8‐oxodeoxyguanosine on the conformation, energetics, and thermodynamic stability of a DNA duplex. Biochemistry 34: 16148 ‐ 16160.
dc.identifier.citedreferencePlum, G.E., Pilch, D.S., Singleton, S.F., and Breslauer, K.J. 1995b. Nucleic acid hybridization: Triplex stability and energetics. Annu. Rev. Biophys. Biomol. Struct. Dyn. 24: 319 ‐ 350.
dc.identifier.citedreferencePlum, G.E., Breslauer, K.J., and Roberts, R.W. 1999. Thermodynamics and kinetics of nucleic acid association/dissociation and folding processes. In Comprehensive Natural Products Chemistry, Vol. 7 ( E.T. Kool, ed.),pp. 15 ‐ 53. Elsevier Science Ltd., Oxford.
dc.identifier.citedreferencePoland, D. 1974. Recursion relation generation of probability profiles for specific‐sequence macromolecules with long range interactions. Biopolymers 13: 1859 ‐ 1871.
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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