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Abstract
This appendix describes the preparation of buffers and reagents used in the manipulation of nucleic acids.RecipesAmmonium acetate, 10 MBCIP, 5% (w/v)DEPC (diethylpyrocarbonate)‐treated solutionsdNTPs: dATP, dTTP, dCTP, and dGTPDTT (dithiothreitol), 1 MEDTA (ethylenediaminetetraacetic acid), 0.5 M (pH 8.0)Ethidium bromide solutionFormamide loading buffer, 2×Gel loading buffer, 6×HCl, 1 MHEPES‐buffered saline, 2×KCl, 1 MKinase buffer, 10×MgCl2, 1 MMgSO4, 1 MNaCl, 5 MNaOH, 10 MNBT (nitroblue tetrazolium chloride), 5% (w/v)PBS (phosphate‐buffered saline)PCR amplification buffer, 10×Phenol, bufferedPhenol/chloroform/isoamyl alcohol, 25:24:1 (v/v/v)PMSF (phenylmethanesulfonyl fluoride), 10 mMPotassium acetate buffer, 0.1 MPotassium phosphate buffer, 0.1 MSDS, 20% (w/v)SDS sample bufferSilanized glasswareSodium acetate, 3 MSodium acetate buffer, 0.1 MSodium phosphate buffer, 0.1 MSSC (sodium chloride/sodium citrate), 20×SSPE (sodium chloride/sodium phosphate/EDTA), 20×TAE (Tris/acetate/EDTA) electrophoresis buffer, 10×TBE (Tris/borate/EDTA) electrophoresis buffer, 10×TBS (Tris‐buffered saline)TCA (trichloroacetic acid), 100% (w/v)TE (Tris/EDTA) bufferTris⋅Cl, 1 MUrea loading buffer, 2×