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Introduction to the Synthesis and Purification of Oligonucleotides
dc.contributor.authorEllington, Andrew
dc.contributor.authorPollard, Jack D.
dc.date.accessioned2018-05-15T20:15:26Z
dc.date.available2018-05-15T20:15:26Z
dc.date.issued2000-02
dc.identifier.citationEllington, Andrew; Pollard, Jack D. (2000). "Introduction to the Synthesis and Purification of Oligonucleotides." Current Protocols in Nucleic Acid Chemistry 00(1): A.3C.1-A.3C.22.
dc.identifier.issn1934-9270
dc.identifier.issn1934-9289
dc.identifier.urihttps://hdl.handle.net/2027.42/143751
dc.description.abstractModern nucleic acid synthesizers utilize phosphite triester chemistries that employ stable phosphoramidite monomers to build a growing polymer. These robust reactions allow easy generation of specific oligodeoxyribo‐ and oligoribonucleotides with a variety of labels, modified linkages, and nonstandard bases. Strategies are given for the maximization of synthetic yield, the generation of sequences containing site‐specific modifications, and the isolation of synthetic oligonucleotides. Protocols describe monitoring the progress of synthesis via the trityl assay and methods for deprotection.
dc.publisherApplied Biosystems
dc.publisherWiley Periodicals, Inc.
dc.titleIntroduction to the Synthesis and Purification of Oligonucleotides
dc.typeArticleen_US
dc.rights.robotsIndexNoFollow
dc.subject.hlbsecondlevelBiological Chemistry
dc.subject.hlbsecondlevelChemical Engineering
dc.subject.hlbsecondlevelPublic Health
dc.subject.hlbsecondlevelChemistry
dc.subject.hlbtoplevelHealth Sciences
dc.subject.hlbtoplevelScience
dc.subject.hlbtoplevelEngineering
dc.description.peerreviewedPeer Reviewed
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/143751/1/cpnca03c.pdf
dc.identifier.doi10.1002/0471142700.nca03cs00
dc.identifier.sourceCurrent Protocols in Nucleic Acid Chemistry
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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