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Analyzing Binding Data
dc.contributor.authorMotulsky, Harvey J.
dc.contributor.authorNeubig, Richard R.
dc.date.accessioned2018-05-15T20:15:36Z
dc.date.available2018-05-15T20:15:36Z
dc.date.issued2010-07
dc.identifier.citationMotulsky, Harvey J.; Neubig, Richard R. (2010). "Analyzing Binding Data." Current Protocols in Neuroscience 52(1): 7.5.1-7.5.65.
dc.identifier.issn1934-8584
dc.identifier.issn1934-8576
dc.identifier.urihttps://hdl.handle.net/2027.42/143761
dc.description.abstractMeasuring the rate and extent of radioligand binding provides information on the number of binding sites, and their affinity and accessibility of these binding sites for various drugs. This unit explains how to design and analyze such experiments. Curr. Protoc. Neurosci. 52:7.5.1‐7.5.65. © 2010 by John Wiley & Sons, Inc.
dc.publisherAcademic Press
dc.publisherWiley Periodicals, Inc.
dc.subject.otherradioligand binding
dc.subject.otherreceptor binding
dc.subject.otherScatchard plot
dc.subject.otherradioligand
dc.subject.otherbinding
dc.subject.otherfluorescence
dc.subject.othercurve fitting
dc.subject.othernonlinear regression
dc.subject.otherBmax
dc.subject.otherKd
dc.subject.otherIC50
dc.subject.othercompetitive binding curve
dc.titleAnalyzing Binding Data
dc.typeArticleen_US
dc.rights.robotsIndexNoFollow
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biology
dc.subject.hlbsecondlevelNeurosciences
dc.subject.hlbsecondlevelPsychology
dc.subject.hlbsecondlevelPublic Health
dc.subject.hlbtoplevelHealth Sciences
dc.subject.hlbtoplevelScience
dc.subject.hlbtoplevelSocial Sciences
dc.description.peerreviewedPeer Reviewed
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/143761/1/cpns0705.pdf
dc.identifier.doi10.1002/0471142301.ns0705s52
dc.identifier.sourceCurrent Protocols in Neuroscience
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dc.identifier.citedreferenceNeubig, R.R. and Cohen, J.B. 1979. Equilibrium binding of [3H]tubocurarine and [3H]acetylcholine by Torpedo postsynaptic membranes: Stoichiometry and ligand interactions. Biochemistry 18: 5464 ‐ 5475.
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dc.identifier.citedreferenceMotulsky, H.J. and Mahan, L.C. 1984. The kinetics of competitive radioligand binding predicted by the law of mass action. Mol. Pharmacol. 25: 1 ‐ 9.
dc.identifier.citedreferenceMcEwen, D.P., Gee, K.R., Kang, H.C., and Neubig, R.R. 2001. Fluorescent BODIPY‐GTP analogs: Real‐time measurement of nucleotide binding to G proteins. Anal. Biochem. 291: 109 ‐ 117.
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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