Salivary exRNA biomarkers to detect gingivitis and monitor disease regression
dc.contributor.author | Kaczor‐urbanowicz, Karolina E. | |
dc.contributor.author | Trivedi, Harsh M. | |
dc.contributor.author | Lima, Patricia O. | |
dc.contributor.author | Camargo, Paulo M. | |
dc.contributor.author | Giannobile, William V. | |
dc.contributor.author | Grogan, Tristan R. | |
dc.contributor.author | Gleber‐netto, Frederico O. | |
dc.contributor.author | Whiteman, Yair | |
dc.contributor.author | Li, Feng | |
dc.contributor.author | Lee, Hyo Jung | |
dc.contributor.author | Dharia, Karan | |
dc.contributor.author | Aro, Katri | |
dc.contributor.author | Martin Carreras‐presas, Carmen | |
dc.contributor.author | Amuthan, Saarah | |
dc.contributor.author | Vartak, Manjiri | |
dc.contributor.author | Akin, David | |
dc.contributor.author | Al‐adbullah, Hiba | |
dc.contributor.author | Bembey, Kanika | |
dc.contributor.author | Klokkevold, Perry R. | |
dc.contributor.author | Elashoff, David | |
dc.contributor.author | Barnes, Virginia M. | |
dc.contributor.author | Richter, Rose | |
dc.contributor.author | DeVizio, William | |
dc.contributor.author | Masters, James G. | |
dc.contributor.author | Wong, David T. W. | |
dc.date.accessioned | 2018-07-13T15:47:35Z | |
dc.date.available | 2019-09-04T20:15:39Z | en |
dc.date.issued | 2018-07 | |
dc.identifier.citation | Kaczor‐urbanowicz, Karolina E. ; Trivedi, Harsh M.; Lima, Patricia O.; Camargo, Paulo M.; Giannobile, William V.; Grogan, Tristan R.; Gleber‐netto, Frederico O. ; Whiteman, Yair; Li, Feng; Lee, Hyo Jung; Dharia, Karan; Aro, Katri; Martin Carreras‐presas, Carmen ; Amuthan, Saarah; Vartak, Manjiri; Akin, David; Al‐adbullah, Hiba ; Bembey, Kanika; Klokkevold, Perry R.; Elashoff, David; Barnes, Virginia M.; Richter, Rose; DeVizio, William; Masters, James G.; Wong, David T. W. (2018). "Salivary exRNA biomarkers to detect gingivitis and monitor disease regression." Journal of Clinical Periodontology 45(7): 806-817. | |
dc.identifier.issn | 0303-6979 | |
dc.identifier.issn | 1600-051X | |
dc.identifier.uri | https://hdl.handle.net/2027.42/144647 | |
dc.description.abstract | AimThis study tests the hypothesis that salivary extracellular RNA (exRNA) biomarkers can be developed for gingivitis detection and monitoring disease regression.Materials and MethodsSalivary exRNA biomarker candidates were developed from a total of 100 gingivitis and nonâ gingivitis individuals using Affymetrix’s expression microarrays. The top 10 differentially expressed exRNAs were tested in a clinical cohort to determine whether the discovered salivary exRNA markers for gingivitis were associated with clinical gingivitis and disease regression. For this purpose, unstimulated saliva was collected from 30 randomly selected gingivitis subjects, the gingival and plaque indexes scores were taken at baseline, 3 and 6Â weeks and salivary exRNAs were assayed by means of reverse transcription quantitative polymerase chain reaction.ResultsEight salivary exRNA biomarkers developed for gingivitis were statistically significantly changed over time, consistent with disease regression. A panel of four salivary exRNAs [SPRR1A, lncâ TET3â 2:1, FAM25A, CRCT1] can detect gingivitis with a clinical performance of 0.91 area under the curve, with 71% sensitivity and 100% specificity.ConclusionsThe clinical values of the developed salivary exRNA biomarkers are associated with gingivitis regression. They offer strong potential to be advanced for definitive validation and clinical laboratory development test. | |
dc.publisher | R Foundation for Statistical Computing | |
dc.publisher | Wiley Periodicals, Inc. | |
dc.subject.other | biomarkers | |
dc.subject.other | exRNA | |
dc.subject.other | gingivitis | |
dc.subject.other | monitoring | |
dc.subject.other | saliva | |
dc.title | Salivary exRNA biomarkers to detect gingivitis and monitor disease regression | |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | |
dc.subject.hlbsecondlevel | Dentistry | |
dc.subject.hlbtoplevel | Health Sciences | |
dc.description.peerreviewed | Peer Reviewed | |
dc.description.bitstreamurl | https://deepblue.lib.umich.edu/bitstream/2027.42/144647/1/jcpe12930.pdf | |
dc.description.bitstreamurl | https://deepblue.lib.umich.edu/bitstream/2027.42/144647/2/jcpe12930_am.pdf | |
dc.identifier.doi | 10.1111/jcpe.12930 | |
dc.identifier.source | Journal of Clinical Periodontology | |
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dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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