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DNA ladders can be used to size polyphosphate resolved by polyacrylamide gel electrophoresis
dc.contributor.authorSmith, Stephanie A.
dc.contributor.authorWang, Yan
dc.contributor.authorMorrissey, James H.
dc.date.accessioned2018-11-20T15:35:29Z
dc.date.available2019-12-02T14:55:09Zen
dc.date.issued2018-10
dc.identifier.citationSmith, Stephanie A.; Wang, Yan; Morrissey, James H. (2018). "DNA ladders can be used to size polyphosphate resolved by polyacrylamide gel electrophoresis." ELECTROPHORESIS 39(19): 2454-2459.
dc.identifier.issn0173-0835
dc.identifier.issn1522-2683
dc.identifier.urihttps://hdl.handle.net/2027.42/146466
dc.description.abstractPAGE is often used to resolve inorganic polyphosphates (polyP), but unfortunately polyP size ladders are not commercially available. Since several dyes that are commonly used to detect nucleic acids in gels also stain polyP, we examined the utility of commercially available DNA size ladders for estimating polyP polymer lengths by gel electrophoresis. Narrow size fractions of polyP were prepared and their polymer lengths were quantified using NMR. Commercially available DNA ladders and these polyP fractions were then subjected to PAGE to determine the relationship between migration of DNA vs polyP, which was found to be: log10(dsDNA length in bp) = 1.66 × log10(polyP length in phosphate units) − 1.97. This relationship between DNA and polyP size held for a variety of different polyacrylamide concentrations, indicating that DNA size ladders can readily be employed to estimate polyP polymer lengths by PAGE.
dc.publisherWiley Periodicals, Inc.
dc.subject.otherPolyphosphate
dc.subject.otherPAGE
dc.subject.otherElectrophoresis
dc.subject.otherDNA ladder
dc.titleDNA ladders can be used to size polyphosphate resolved by polyacrylamide gel electrophoresis
dc.typeArticleen_US
dc.rights.robotsIndexNoFollow
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biology
dc.subject.hlbsecondlevelChemical Engineering
dc.subject.hlbsecondlevelChemistry
dc.subject.hlbsecondlevelMaterials Science and Engineering
dc.subject.hlbtoplevelEngineering
dc.subject.hlbtoplevelScience
dc.subject.hlbtoplevelHealth Sciences
dc.description.peerreviewedPeer Reviewed
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/146466/1/elps6690_am.pdf
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/146466/2/elps6690.pdf
dc.identifier.doi10.1002/elps.201800227
dc.identifier.sourceELECTROPHORESIS
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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