Intramuscular inflammatory and resolving lipid profile responses to an acute bout of resistance exercise in men
Vella, Luke; Markworth, James F.; Farnfield, Michelle M.; Maddipati, Krishna R.; Russell, Aaron P.; Cameron‐smith, David
2019-08
Citation
Vella, Luke; Markworth, James F.; Farnfield, Michelle M.; Maddipati, Krishna R.; Russell, Aaron P.; Cameron‐smith, David (2019). "Intramuscular inflammatory and resolving lipid profile responses to an acute bout of resistance exercise in men." Physiological Reports (13): n/a-n/a.
Abstract
Lipid mediators including classical arachidonic acidâ derived eicosanoids (e.g. prostaglandins and leukotrienes) and more recently identified specialized proâ resolvingâ mediator metabolites of the omegaâ 3 fatty acids play essential roles in initiation, selfâ limitation, and active resolution of acute inflammatory responses. In this study, we examined the bioactive lipid mediator profile of human skeletal muscle at rest and following acute resistance exercise. Twelve male subjects completed a single bout of maximal isokinetic unilateral knee extension exercise and muscle biopsies were taken from the m.vastus lateralis before and at 2, 4, and 24 h of recovery. Muscle tissue lipid mediator profile was analyzed via liquid chromatographyâ mass spectrometry (LCâ MS)â based targeted lipidomics. At 2 h postexercise, there was an increased intramuscular abundance of cyclooxygenase (COX)â derived thromboxanes (TXB2: 3.33 fold) and prostaglandins (PGE2: 2.52 fold and PGF2α: 1.77 fold). Resistance exercise also transiently increased muscle concentrations of lipoxygenase (LOX) pathwayâ derived leukotrienes (12â Oxo LTB4: 1.49 fold and 20â COOH LTB4: 2.91 fold), monohydroxyâ eicosatetraenoic acids (5â HETE: 2.66 fold, 12â HETE: 2.83 fold, and 15â HETE: 1.69 fold) and monohydroxyâ docosahexaenoic acids (4â HDoHE: 1.69 fold, 7â HDoHE: 1.58 fold and 14â HDoHE: 2.35 fold). Furthermore, the abundance of CYP pathwayâ derived epoxyâ and dihydroxyâ eicosatrienoic acids was increased in 2 h postexercise biopsies (5,6â EpETrE: 2.48 fold, 11,12â DiHETrE: 1.66 fold and 14,15â DiHETrE: 2.23 fold). These data reveal a range of bioactive lipid mediators as present within human skeletal muscle tissue and demonstrate that acute resistance exercise transiently stimulates the local production of both proinflammatory eicosanoids and pathway markers in specialized proresolving mediator biosynthesis circuits.Proresolving fatty acid metabolites are essential in regulation of acute inflammatory responses. In response to a single bout of maximal isokinetic unilateral knee extension exercise muscle lipid mediator profile was characterized by transient local production of both proinflammatory and proresolving mediators. These included the identification of derivatives of the 5â LOX (5â HETE, 4â HDoHE and 7â HDoHE), 12â LOX (12â HETE and tetranor 12â HETE) and 15â LOX (15â HETE) pathways.Publisher
Wiley Periodicals, Inc.
ISSN
2051-817X 2051-817X
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