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Characterization of macrophage polarization in periodontal disease
dc.contributor.authorGaraicoa‐pazmino, Carlos
dc.contributor.authorFretwurst, Tobias
dc.contributor.authorSquarize, Cristiane H.
dc.contributor.authorBerglundh, Tord
dc.contributor.authorGiannobile, William V.
dc.contributor.authorLarsson, Lena
dc.contributor.authorCastilho, Rogerio M.
dc.date.accessioned2019-08-09T17:12:55Z
dc.date.availableWITHHELD_13_MONTHS
dc.date.available2019-08-09T17:12:55Z
dc.date.issued2019-08
dc.identifier.citationGaraicoa‐pazmino, Carlos ; Fretwurst, Tobias; Squarize, Cristiane H.; Berglundh, Tord; Giannobile, William V.; Larsson, Lena; Castilho, Rogerio M. (2019). "Characterization of macrophage polarization in periodontal disease." Journal of Clinical Periodontology 46(8): 830-839.
dc.identifier.issn0303-6979
dc.identifier.issn1600-051X
dc.identifier.urihttps://hdl.handle.net/2027.42/150506
dc.description.abstractAimTo explore the M1/M2 status of macrophage polarization from healthy, gingivitis, and periodontitis patient samples.Materials and methodsGingival biopsies were collected from 42 individuals (14 gingivitis, 18 periodontitis, and 10 healthy samples) receiving periodontal therapy. Histomorphology analysis was performed with haematoxylin and eosin staining. Immunofluorescence was performed using a combination of CD68 (macrophages), iNOS (M1), and CD206 (M2) in order to acquire changes in macrophage polarization at a singleâ cell resolution. Macrophages were quantified under microscopy using narrow wavelength filters to detect Alexa 488, Alexa 568, Alexa 633 fluorophores, and Hoechst 33342 to identify cellular DNA content.ResultsGingivitis and periodontitis samples showed higher levels of macrophages compared with healthy samples. Unexpectedly, periodontitis samples displayed lower levels of macrophages dispersed in the stromal tissues compared with gingivitis samples; however, it remained higher than healthy tissues. The polarization of macrophages appears to be reduced in periodontitis and showed similar levels to those observed in healthy tissues.ConclusionsOur study found that gingivitis and periodontitis differ from each other by the levels of macrophage infiltrate, but not by changes in macrophage polarization.
dc.publisherWiley Periodicals, Inc.
dc.subject.otherperiodontitis
dc.subject.othergingivitis
dc.subject.otherallergy and immunology
dc.subject.otherimmunohistochemistry
dc.subject.otherimmunologic factors
dc.subject.otherinflammation
dc.titleCharacterization of macrophage polarization in periodontal disease
dc.typeArticle
dc.rights.robotsIndexNoFollow
dc.subject.hlbsecondlevelDentistry
dc.subject.hlbtoplevelHealth Sciences
dc.description.peerreviewedPeer Reviewed
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/150506/1/jcpe13156_am.pdf
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/150506/2/jcpe13156.pdf
dc.identifier.doi10.1111/jcpe.13156
dc.identifier.sourceJournal of Clinical Periodontology
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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