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Whole‐cell biocatalysis platform for gram‐scale oxidative dearomatization of phenols

dc.contributor.authorBaker Dockrey, Summer A.
dc.contributor.authorDoyon, Tyler J.
dc.contributor.authorPerkins, Jonathan C.
dc.contributor.authorNarayan, Alison R. H.
dc.date.accessioned2019-08-09T17:13:59Z
dc.date.availableWITHHELD_11_MONTHS
dc.date.available2019-08-09T17:13:59Z
dc.date.issued2019-06
dc.identifier.citationBaker Dockrey, Summer A.; Doyon, Tyler J.; Perkins, Jonathan C.; Narayan, Alison R. H. (2019). "Whole‐cell biocatalysis platform for gram‐scale oxidative dearomatization of phenols." Chemical Biology & Drug Design (6): 1207-1213.
dc.identifier.issn1747-0277
dc.identifier.issn1747-0285
dc.identifier.urihttps://hdl.handle.net/2027.42/150545
dc.description.abstractTechnologies enabling new enzyme discovery and efficient protein engineering have spurred intense interest in the development of biocatalytic reactions. In recent years, whole‐cell biocatalysis has received attention as a simple, efficient, and scalable biocatalytic reaction platform. Inspired by these developments, we have established a whole‐cell protocol for oxidative dearomatization of phenols using the flavin‐dependent monooxygenase, TropB. This approach provides a scalable biocatalytic platform for accessing gram‐scale quantities of chiral synthetic building blocks.The development of scalable and economical biocatalytic reaction platforms is critical for the application of biocatalysis in synthetic chemistry. Therefore, we have established a whole‐cell method for the oxidative dearomatization of phenols using the flavin‐dependent monooxygenase, TropB. In comparison with reactions using isolated enzyme, our whole‐cell method allowed us to perform tenfold more reactions per liter of cell culture, without loss of site‐ or stereoselectivity.
dc.publisherWiley Periodicals, Inc.
dc.subject.otherwhole‐cell reactions
dc.subject.otherbiocatalysis
dc.subject.otherflavin‐dependent monooxygenase
dc.subject.otheroxidative dearomatization
dc.subject.otherpreparative‐scale
dc.titleWhole‐cell biocatalysis platform for gram‐scale oxidative dearomatization of phenols
dc.typeArticle
dc.rights.robotsIndexNoFollow
dc.subject.hlbsecondlevelPharmacy and Pharmacology
dc.subject.hlbtoplevelHealth Sciences
dc.description.peerreviewedPeer Reviewed
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/150545/1/cbdd13443.pdf
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/150545/2/cbdd13443_am.pdf
dc.identifier.doi10.1111/cbdd.13443
dc.identifier.sourceChemical Biology & Drug Design
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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