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The Unmixing Problem: A Guide to Applying Single‐Cell RNA Sequencing to Bone
dc.contributor.authorGreenblatt, Matthew B
dc.contributor.authorOno, Noriaki
dc.contributor.authorAyturk, Ugur M
dc.contributor.authorDebnath, Shawon
dc.contributor.authorLalani, Sarfaraz
dc.date.accessioned2019-08-09T17:14:32Z
dc.date.availableWITHHELD_12_MONTHS
dc.date.available2019-08-09T17:14:32Z
dc.date.issued2019-07
dc.identifier.citationGreenblatt, Matthew B; Ono, Noriaki; Ayturk, Ugur M; Debnath, Shawon; Lalani, Sarfaraz (2019). "The Unmixing Problem: A Guide to Applying Single‐Cell RNA Sequencing to Bone." Journal of Bone and Mineral Research 34(7): 1207-1219.
dc.identifier.issn0884-0431
dc.identifier.issn1523-4681
dc.identifier.urihttps://hdl.handle.net/2027.42/150567
dc.description.abstractBone is composed of a complex mixture of many dynamic cell types. Flow cytometry and in vivo lineage tracing have offered early progress toward deconvoluting this heterogeneous mixture of cells into functionally well‐defined populations suitable for further studies. Single‐cell sequencing is poised as a key complementary technique to better understand the cellular basis of bone metabolism and development. However, single‐cell sequencing approaches still have important limitations, including transcriptional effects of cell isolation and sparse sampling of the transcriptome, that must be considered during experimental design and analysis to harness the power of this approach. Accounting for these limitations requires a deep knowledge of the tissue under study. Therefore, with the emergence of accessible tools for conducting and analyzing single‐cell RNA sequencing (scRNA‐seq) experiments, bone biologists will be ideal leaders in the application of scRNA‐seq to the skeleton. Here we provide an overview of the steps involved with a single‐cell sequencing analysis of bone, focusing on practical considerations needed for a successful study. © 2019 American Society for Bone and Mineral Research.
dc.publisherWiley Periodicals, Inc.
dc.subject.otherSINGLE‐CELL RNA SEQUENCING
dc.subject.otherOSTEOBLASTS
dc.subject.otherMESENCHYMAL STEM CELLS
dc.titleThe Unmixing Problem: A Guide to Applying Single‐Cell RNA Sequencing to Bone
dc.typeArticle
dc.rights.robotsIndexNoFollow
dc.subject.hlbsecondlevelInternal Medicine and Specialities
dc.subject.hlbtoplevelHealth Sciences
dc.description.peerreviewedPeer Reviewed
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/150567/1/jbmr3802_am.pdf
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/150567/2/jbmr3802.pdf
dc.identifier.doi10.1002/jbmr.3802
dc.identifier.sourceJournal of Bone and Mineral Research
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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