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Genetic construct schematic to generate a conditional allele to knock out the Aldh1a2 gene in mice

dc.contributor.authorCheung, Leonard Yan Ming
dc.contributor.authorCamper, Sally
dc.contributor.authorNapoli, Joseph
dc.date.accessioned2019-10-02T17:48:03Z
dc.date.available2019-10-02T17:48:03Z
dc.date.issued2019-10-02
dc.identifier.urihttps://hdl.handle.net/2027.42/151759
dc.descriptionGeneration of a conditional allele, via a sequence replacement strategy to knock-out the Aldh1a2 gene. The construct contains loxP sites that flank exon 4, a 2.5 kb 5’ short arm of homology, a 5.6 kb 3’ long arm of homology, a Diphtheria Toxin A (DTA) cassette, and a Neomycin (Neo) cassette flanked by frt sites for selective deletion. The Neo element will allow for positive selection in ES cells, while the DTA element will permit negative selection in ES cells. After homologous recombination of our conditional knock-out construct, the Aldh1a2 gene will have normal expression until Cre-mediated deletion of exon 4. Deletion of exon 4 creates a frameshift mutation and a premature stop, which should render the Aldh1a2 gene inactive.en_US
dc.description.abstractConditional-ready mice carrying loxP sites flanking exon 4 of mouse Aldh1a2 on chromosome 9 were designed by Joseph Napoli (UC Berkeley) and generated at UC Davis.en_US
dc.description.sponsorshipDr. Napoli was supported by NIH DK112754.en_US
dc.language.isoen_USen_US
dc.subjectAldh1a2 floxen_US
dc.subjectAldh1a2en_US
dc.subjectretinoic aciden_US
dc.subjectCre LoxPen_US
dc.subjectconditional mouseen_US
dc.titleGenetic construct schematic to generate a conditional allele to knock out the Aldh1a2 gene in miceen_US
dc.typeImageen_US
dc.subject.hlbsecondlevelHuman Genetics
dc.subject.hlbtoplevelHealth Sciences
dc.contributor.affiliationumHuman Genetics, Department ofen_US
dc.contributor.affiliationotherDepartment of Nutritional Sciences and Toxicology, University of California, Berkeleyen_US
dc.contributor.affiliationumcampusAnn Arboren_US
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/151759/1/aldh1a2_construct.tif
dc.description.filedescriptionDescription of aldh1a2_construct.tif : Figure: Schematic of genetic construct to generate mice to conditionally delete Aldh1a2.
dc.owningcollnameHuman Genetics, Department of


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