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Magnetic Alignment of Polymer Macro‐Nanodiscs Enables Residual‐Dipolar‐Coupling‐Based High‐Resolution Structural Studies by NMR Spectroscopy
dc.contributor.authorRavula, Thirupathi
dc.contributor.authorRamamoorthy, Ayyalusamy
dc.date.accessioned2019-10-30T15:29:42Z
dc.date.availableWITHHELD_13_MONTHS
dc.date.available2019-10-30T15:29:42Z
dc.date.issued2019-10-14
dc.identifier.citationRavula, Thirupathi; Ramamoorthy, Ayyalusamy (2019). "Magnetic Alignment of Polymer Macro‐Nanodiscs Enables Residual‐Dipolar‐Coupling‐Based High‐Resolution Structural Studies by NMR Spectroscopy." Angewandte Chemie 131(42): 15067-15070.
dc.identifier.issn0044-8249
dc.identifier.issn1521-3757
dc.identifier.urihttps://hdl.handle.net/2027.42/151827
dc.description.abstractExperimentally measured residual dipolar couplings (RDCs) are highly valuable for atomic‐resolution structural and dynamic studies of molecular systems ranging from small molecules to large proteins by solution NMR spectroscopy. Here we demonstrate the first use of magnetic‐alignment behavior of lyotropic liquid‐crystalline polymer macro‐nanodiscs (>20 nm in diameter) as a novel alignment medium for the measurement of RDCs using high‐resolution NMR. The easy preparation of macro‐nanodiscs, their high stability against pH changes and the presence of divalent metal ions, and their high homogeneity make them an efficient tool to investigate a wide range of molecular systems including natural products, proteins, and RNA.Korrekt ausgerichtet: Polymer‐Nanoscheiben werden als Ausrichtungsmedium für die Messung dipolarer Restkopplungen mit hochauflösender NMR‐Spektroskopie genutzt. Ihre einfache Herstellung, hohe Stabilität gegen pH‐Änderungen und die Anwesenheit zweiwertiger Metallionen sowie ihre hohe Homogenität machen sie zu einem effizienten Werkzeug für die Untersuchung vieler molekularer Systeme wie Naturstoffe, Proteine und RNA.
dc.publisherWiley Periodicals, Inc.
dc.subject.otherMagnetische Ausrichtung
dc.subject.otherCytochrom c
dc.subject.otherNMR-Spektroskopie
dc.subject.otherPolymer-Nanoscheiben
dc.subject.otherDipolare Restkopplung
dc.titleMagnetic Alignment of Polymer Macro‐Nanodiscs Enables Residual‐Dipolar‐Coupling‐Based High‐Resolution Structural Studies by NMR Spectroscopy
dc.typeArticle
dc.rights.robotsIndexNoFollow
dc.subject.hlbsecondlevelChemical Engineering
dc.subject.hlbsecondlevelChemistry
dc.subject.hlbsecondlevelMaterials Science and Engineering
dc.subject.hlbtoplevelEngineering
dc.subject.hlbtoplevelScience
dc.description.peerreviewedPeer Reviewed
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/151827/1/ange201907655-sup-0001-misc_information.pdf
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/151827/2/ange201907655_am.pdf
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/151827/3/ange201907655.pdf
dc.identifier.doi10.1002/ange.201907655
dc.identifier.sourceAngewandte Chemie
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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