View Item 

Show simple item record

Synthesis of Oligoribonucleotides Using the 2‐Nitrobenzyloxymethyl Group for 2′‐Hydroxyl Protection

dc.contributor.authorMiller, Tod J.
dc.contributor.authorSchwartz, Miriam E.
dc.contributor.authorGough, Geoffrey R.
dc.date.accessioned2020-01-13T15:02:53Z
dc.date.available2020-01-13T15:02:53Z
dc.date.issued2000-12
dc.identifier.citationMiller, Tod J.; Schwartz, Miriam E.; Gough, Geoffrey R. (2000). "Synthesis of Oligoribonucleotides Using the 2‐Nitrobenzyloxymethyl Group for 2′‐Hydroxyl Protection." Current Protocols in Nucleic Acid Chemistry 3(1): 3.7.1-3.7.8.
dc.identifier.issn1934-9270
dc.identifier.issn1934-9289
dc.identifier.urihttps://hdl.handle.net/2027.42/152494
dc.description.abstractRNA can be rapidly synthesized using protected ribonucleoside phosphoramidite monomers that carry 2‐nitrobenzyloxymethyl groups on their 2‐hydroxyls. These protecting groups are removed from the oligoribonucleotide products by exposure to long‐wave UV light.
dc.publisherWiley Periodicals, Inc.
dc.titleSynthesis of Oligoribonucleotides Using the 2‐Nitrobenzyloxymethyl Group for 2′‐Hydroxyl Protection
dc.typeArticle
dc.rights.robotsIndexNoFollow
dc.subject.hlbsecondlevelBiological Chemistry
dc.subject.hlbsecondlevelChemical Engineering
dc.subject.hlbsecondlevelChemistry
dc.subject.hlbsecondlevelPublic Health
dc.subject.hlbtoplevelScience
dc.subject.hlbtoplevelEngineering
dc.subject.hlbtoplevelHealth Sciences
dc.description.peerreviewedPeer Reviewed
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/152494/1/cpnc0307.pdf
dc.identifier.doi10.1002/0471142700.nc0307s03
dc.identifier.sourceCurrent Protocols in Nucleic Acid Chemistry
dc.identifier.citedreferenceSinha, N.D., Biernat, J., McManus, J., and Köster, H. 1984. Polymer support oligonucleotide synthesis XVIII: Use of β‐cyanoethyl‐ N, N ‐dialkylamino‐/ N ‐morpholino phosphoramidite of deoxynucleosides for the synthesis of DNA fragments simplifying deprotection and isolation of the final product. Nucl. Acids Res. 12: 4539 ‐ 4557.
dc.identifier.citedreferenceVinayak, R., Colonna, F., Tsou, D., Mullah, B., Andrus, A., and Sproat, B. 1994. arge scale chemical synthesis and purification of RNA. Nucl. Acids Symp. Ser. 31: 165 – 166
dc.identifier.citedreferenceSchwartz, M.E., Breaker, R.R., Asteriadis, G.T., deBear, J.S., and Gough, G.R. 1992. Rapid synthesis of oligoribonucleotides using 2′‐ O ‐(2‐ nitrobenzyloxymethyl)‐protected monomers. Bio. Med. Chem. Lett. 2: 1019 ‐ 1024.
dc.identifier.citedreferenceSchwartz, M.E., Breaker, R.R., Asteriadis, G.T., deBear, J.S., and Gough, G.R. 1992. Rapid synthesis of oligoribonucleotides using 2′‐ O ‐(2‐nitrobenzyloxymethyl)‐protected monomers. BioMed. Chem. Lett. 2: 1019 ‐ 1024.
dc.identifier.citedreferenceSchwartz, M.E., Breaker, R.R., Asteriadis, G.T., and Gough, G.R. 1995. A universal adapter for chemical synthesis of DNA or RNA on any single type of solid support. Tetrahedron Lett. 36: 27 ‐ 30.
dc.owningcollnameInterdisciplinary and Peer-Reviewed


Files in this item

Name:
cpnc0307.pdf
Size:
159.2KB
Format:
PDF
View/Open

Show simple item record

Remediation of Harmful Language

The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.

Accessibility

If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.