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Capillary Electrophoresis of DNA
dc.contributor.authorSmith, Alan
dc.contributor.authorNelson, Robert J.
dc.date.accessioned2020-01-13T15:04:10Z
dc.date.available2020-01-13T15:04:10Z
dc.date.issued2003-06
dc.identifier.citationSmith, Alan; Nelson, Robert J. (2003). "Capillary Electrophoresis of DNA." Current Protocols in Nucleic Acid Chemistry 13(1): 10.9.1-10.9.16.
dc.identifier.issn1934-9270
dc.identifier.issn1934-9289
dc.identifier.urihttps://hdl.handle.net/2027.42/152538
dc.description.abstractCapillary electrophoresis (CE) is an alternative to conventional slab gel electrophoresis for the separation of DNA fragments. CE offers a number of advantages over slab gel separations in terms of speed, resolution, sensitivity, and data handling. Separation times are generally only a few minutes and the DNA is detected either by UV absorption or by fluorescent labeling. The quantity of DNA required for separation is in the nanogram range. Single‐base resolution can be obtained on fragments up to several hundred base pairs. In the presence of appropriate standards, fragments can be accurately sized based on relative electrophoretic mobility. A protocol for the analysis of synthetic oligonucleotides in a flowable matrix is described in this unit.
dc.publisherJohn Wiley & Sons
dc.titleCapillary Electrophoresis of DNA
dc.typeArticle
dc.rights.robotsIndexNoFollow
dc.subject.hlbsecondlevelBiological Chemistry
dc.subject.hlbsecondlevelChemical Engineering
dc.subject.hlbsecondlevelChemistry
dc.subject.hlbsecondlevelPublic Health
dc.subject.hlbtoplevelScience
dc.subject.hlbtoplevelEngineering
dc.subject.hlbtoplevelHealth Sciences
dc.description.peerreviewedPeer Reviewed
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/152538/1/cpnc1009.pdf
dc.identifier.doi10.1002/0471142700.nc1009s13
dc.identifier.sourceCurrent Protocols in Nucleic Acid Chemistry
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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