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Synthesis of 5′‐O‐Phosphoramidites with a Photolabile 3′‐O‐Protecting Group
dc.contributor.authorBeier, Markus
dc.contributor.authorHoheisel, Jörg D.
dc.date.accessioned2020-01-13T15:04:58Z
dc.date.available2020-01-13T15:04:58Z
dc.date.issued2004-06
dc.identifier.citationBeier, Markus; Hoheisel, Jörg D. (2004). "Synthesis of 5′‐O‐Phosphoramidites with a Photolabile 3′‐O‐Protecting Group." Current Protocols in Nucleic Acid Chemistry 17(1): 12.3.1-12.3.10.
dc.identifier.issn1934-9270
dc.identifier.issn1934-9289
dc.identifier.urihttps://hdl.handle.net/2027.42/152566
dc.description.abstractThis unit describes the chemical synthesis of phosphoramidite building blocks that carry a protecting group at the 3′ position. These inversely oriented synthons expose a 2‐(2‐nitrophenyl)propoxycarbonyl (NPPOC) group as the photolabile protecting group of choice. Among other applications, the building blocks can be employed for light‐controlled in situ synthesis of DNA microarrays, producing arrayed oligonucleotides that are attached to the support via their 5′ ends, leaving their 3′ termini available to act as substrates for polymerases.
dc.publisherWiley Periodicals, Inc.
dc.subject.otherDNA microarray
dc.subject.otherphotolabile protecting group
dc.subject.otherphosphoramidite
dc.titleSynthesis of 5′‐O‐Phosphoramidites with a Photolabile 3′‐O‐Protecting Group
dc.typeArticle
dc.rights.robotsIndexNoFollow
dc.subject.hlbsecondlevelBiological Chemistry
dc.subject.hlbsecondlevelChemical Engineering
dc.subject.hlbsecondlevelChemistry
dc.subject.hlbsecondlevelPublic Health
dc.subject.hlbtoplevelScience
dc.subject.hlbtoplevelEngineering
dc.subject.hlbtoplevelHealth Sciences
dc.description.peerreviewedPeer Reviewed
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/152566/1/cpnc1203.pdf
dc.identifier.doi10.1002/0471142700.nc1203s17
dc.identifier.sourceCurrent Protocols in Nucleic Acid Chemistry
dc.identifier.citedreferenceBeier, M., Stephan, A., and Hoheisel, J.D. 2001. Synthesis of photolabile 5′‐ O ‐phosphoramidites for the production of microarrays of inversely oriented oligonucleotides. Helv. Chim. Acta 84: 2089 ‐ 2095.
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dc.identifier.citedreferenceBeier, M. and Hoheisel, J.D. 2000. Production by quantitative photolithographic synthesis of individually quality checked DNA microarrays. Nucl. Acids Res. 28: e11.
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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