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Production of a Heterozygous Mutant Cell Line by Homologous Recombination (Single Knockout)

dc.contributor.authorMortensen, Richard
dc.date.accessioned2020-01-13T15:05:06Z
dc.date.available2020-01-13T15:05:06Z
dc.date.issued2011-04
dc.identifier.citationMortensen, Richard (2011). "Production of a Heterozygous Mutant Cell Line by Homologous Recombination (Single Knockout)." Current Protocols in Neuroscience 55(1): 4.30.1-4.30.12.
dc.identifier.issn1934-8584
dc.identifier.issn1934-8576
dc.identifier.urihttps://hdl.handle.net/2027.42/152573
dc.description.abstractGene targeting by homologous recombination is a powerful and widely used technique for introduction of specific gene mutations (frequently a gene inactivation) in transgenic animals. The basic method detailed in this unit uses sequences homologous to the endogenous gene flanking the mutation. While methods using bacterial artificial chromosomes (BACs) and recombineering may be used, in most cases simpler bacterial plasmid clones with several kb of homology are sufficient. This protocol details the strategic factors in designing the constructs for selection and screening for homologous recombination. Curr. Protoc. Neurosci. 55:4.30.1‐4.30.12. © 2011 by John Wiley & Sons, Inc.
dc.publisherIRL Press
dc.publisherWiley Periodicals, Inc.
dc.subject.othermutation
dc.subject.otherheterozygous mouse
dc.subject.otherhomologous recombination
dc.subject.othersingle knockout
dc.titleProduction of a Heterozygous Mutant Cell Line by Homologous Recombination (Single Knockout)
dc.typeArticle
dc.rights.robotsIndexNoFollow
dc.subject.hlbsecondlevelPublic Health
dc.subject.hlbsecondlevelPsychology
dc.subject.hlbsecondlevelNeurosciences
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biology
dc.subject.hlbtoplevelScience
dc.subject.hlbtoplevelHealth Sciences
dc.subject.hlbtoplevelSocial Sciences
dc.description.peerreviewedPeer Reviewed
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/152573/1/cpns0430.pdf
dc.identifier.doi10.1002/0471142301.ns0430s55
dc.identifier.sourceCurrent Protocols in Neuroscience
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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