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Cartridge Methods for Oligonucleotide Purification
dc.contributor.authorAndrus, Alex
dc.contributor.authorKuimelis, Robert G.
dc.date.accessioned2020-01-13T15:06:17Z
dc.date.available2020-01-13T15:06:17Z
dc.date.issued2000-07
dc.identifier.citationAndrus, Alex; Kuimelis, Robert G. (2000). "Cartridge Methods for Oligonucleotide Purification." Current Protocols in Nucleic Acid Chemistry 1(1): 10.7.1-10.7.5.
dc.identifier.issn1934-9270
dc.identifier.issn1934-9289
dc.identifier.urihttps://hdl.handle.net/2027.42/152627
dc.description.abstractProtocols are given for purification of oligonucleotides by dimethoxytrityl‐sensitive and affinity desalting methods. The protocols are applicable for many of the convenient disposable products available for rapid oligonucleotide purification, clean‐up by selective adsorption, and elution on solid‐phase media. Many of these products are prepackaged, single‐use cartridges or columns filled with affinity or size‐exclusion media.
dc.publisherWiley Periodicals, Inc.
dc.titleCartridge Methods for Oligonucleotide Purification
dc.typeArticle
dc.rights.robotsIndexNoFollow
dc.subject.hlbsecondlevelBiological Chemistry
dc.subject.hlbsecondlevelChemical Engineering
dc.subject.hlbsecondlevelChemistry
dc.subject.hlbsecondlevelPublic Health
dc.subject.hlbtoplevelScience
dc.subject.hlbtoplevelEngineering
dc.subject.hlbtoplevelHealth Sciences
dc.description.peerreviewedPeer Reviewed
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/152627/1/cpnc1007.pdf
dc.identifier.doi10.1002/0471142700.nc1007s01
dc.identifier.sourceCurrent Protocols in Nucleic Acid Chemistry
dc.identifier.citedreferenceMcBride, L., McCollum, C., Davidson, S., Efcavitch, J.W., Andrus, A., and Lombardi, S. 1988. A new reliable cartridge for the rapid purification of synthetic DNA. Biotechniques 6: 362 ‐ 367.
dc.identifier.citedreferenceMullah, B. and Andrus, A. 1996. Purification of 5′‐ O ‐trityl‐On oligoribonucleotides. Investigation of phosphate migration during purification and detritylation. Nucleosides Nucleotides 15: 419 ‐ 430.
dc.identifier.citedreferenceApplied Biosystems. 1991. New Applications for the Oligonucleotide Purification Cartridge. User Bulletin No. 59.
dc.identifier.citedreferenceBlocker, H., Frank, R., Heisterberg‐Moutsis, G., Kurth, G., and Meyerhans, A. 1991. Improved Process for the Purification of Synthetic Oligonucleotides. U.S. Patent No. 4,997,927.
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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