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Overview of Gene Targeting by Homologous Recombination

dc.contributor.authorMortensen, Richard
dc.date.accessioned2020-01-13T15:06:41Z
dc.date.available2020-01-13T15:06:41Z
dc.date.issued2007-07
dc.identifier.citationMortensen, Richard (2007). "Overview of Gene Targeting by Homologous Recombination." Current Protocols in Neuroscience 40(1): 4.29.1-4.29.13.
dc.identifier.issn1934-8584
dc.identifier.issn1934-8576
dc.identifier.urihttps://hdl.handle.net/2027.42/152646
dc.description.abstractThe analysis of mutant organisms and cell lines is important in determining the function of specific proteins. Recent technological advances in gene targeting by homologous recombination in mammalian systems enable the production of mutants in any desired gene, and can be used to produce mutant mouse strains and mutant cell lines. The yeast Flp/FRT recombinase system and bacteriophage recombinases such as Cre and its recognition sequence, loxP, allow spatial and temporal control of knockouts. This unit discusses crucial issues for homologous recombination experiments, including requirements for the source of DNA, criteria for the targeting constructs, methods of enrichment for homologous recombinants, (positive and negative selection, and the use of endogenous promoters), and the types of mutations that can be created. Curr. Protoc. Neurosci. 40:4.29.1‐4.29.13. © by John Wiley & Sons, Inc.
dc.publisherWiley Periodicals, Inc.
dc.subject.otherphenotypic selection
dc.subject.othermutation
dc.subject.otherFlP/FRT
dc.subject.otherrecombinase
dc.subject.othermammalian
dc.subject.otherhomologous recombination
dc.subject.otherCre‐loxP
dc.titleOverview of Gene Targeting by Homologous Recombination
dc.typeArticle
dc.rights.robotsIndexNoFollow
dc.subject.hlbsecondlevelPublic Health
dc.subject.hlbsecondlevelPsychology
dc.subject.hlbsecondlevelNeurosciences
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biology
dc.subject.hlbtoplevelSocial Sciences
dc.subject.hlbtoplevelHealth Sciences
dc.subject.hlbtoplevelScience
dc.description.peerreviewedPeer Reviewed
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/152646/1/cpns0429.pdf
dc.identifier.doi10.1002/0471142301.ns0429s40
dc.identifier.sourceCurrent Protocols in Neuroscience
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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