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Development of Molecular Genetic Interventions for HIV Infection
dc.contributor.authorWoffendin, Clive
dc.contributor.authorRanga, Udaykumar
dc.contributor.authorNabel, Gary J.
dc.date.accessioned2020-01-13T15:07:50Z
dc.date.available2020-01-13T15:07:50Z
dc.date.issued1997-02
dc.identifier.citationWoffendin, Clive; Ranga, Udaykumar; Nabel, Gary J. (1997). "Development of Molecular Genetic Interventions for HIV Infection." Current Protocols in Human Genetics 12(1): 13.6.1-13.6.18.
dc.identifier.issn1934-8266
dc.identifier.issn1934-8258
dc.identifier.urihttps://hdl.handle.net/2027.42/152699
dc.description.abstractThis unit includes a set of protocols for the ex vivo transfer of genes into CD4+ T cells, to be used in the initial evaluation of genes protecting against HIV infection in gene therapy protocols. The describes isolating and expanding CD4+ T cells from the patient. The cells are then transduced by either retroviral transduction or particle‐mediated gene transfer and reinfused into the patient. To monitor the effectiveness of gene transfer, genomic DNA is prepared from the patient’s cells. Detection of vector DNA by PCR analysis of the patient’s genomic DNA following gene transfer is also described in detail.
dc.publisherJohn Wiley & Sons
dc.titleDevelopment of Molecular Genetic Interventions for HIV Infection
dc.typeArticle
dc.rights.robotsIndexNoFollow
dc.subject.hlbsecondlevelBiological Chemistry
dc.subject.hlbsecondlevelGenetics
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biology
dc.subject.hlbtoplevelHealth Sciences
dc.subject.hlbtoplevelScience
dc.description.peerreviewedPeer Reviewed
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/152699/1/cphg1306.pdf
dc.identifier.doi10.1002/0471142905.hg1306s12
dc.identifier.sourceCurrent Protocols in Human Genetics
dc.identifier.citedreferenceLeavitt, M.C., Yu, M., Yamada, O., Kraus, G., Looney, G., Poeschla, E., Wong‐Staal, F. 1994. Transfer of an anti‐HIV‐1 ribozyme gene into primary human lymphocytes. Hum. Gene Ther. 5(9): 1115 ‐ 1120.
dc.identifier.citedreferenceLevine, L.L., Mosca, J.D., Riley, J.L., Carroll, R.G., Vahey, M.T., Jagodzinski, L.L., Wagner, K.F., Mayers, D.L., Burke, D.S., Weislow, O.S., St. Louis, D.C., and June, C.H. 1996. Antiviral effect and ex vivo CD4 T cell proliferation in HIV positive patients as a result of CD28 costimulation. Science 272: 1939 ‐ 1943.
dc.identifier.citedreferenceNabel, G.J., Gene therapy approaches to AIDS. 1994. AIDS 8 (suppl. 1): S61 ‐ 69.
dc.identifier.citedreferenceNabel, G.J., Fox, B.A., Post, L., Thompson, C.B., and Woffendin, C. 1994. A molecular genetic intervention for AIDS‐effects of a transdominant negative form of Rev. Hum. Gene Ther. 5: 79 ‐ 92.
dc.identifier.citedreferencePoli, G. and Fauci, A.S. 1993. General guidelines for experimenting with HIV. In Current Protocols in Immunology ( J.E. Coligan, A.M. Kruisbeek, D.H. Margulies, E.M. Shevach, and W. Strober, eds.)pp. 12.1.1 ‐ 12.1.7. John Wiley & Sons, New York.
dc.identifier.citedreferenceSanford, J.C., Smith, F. D., and Russell, J.A. 1993. Optimizing the biolistic process for different biological applications. Methods Enzymol. 217: 483 ‐ 509.
dc.identifier.citedreferenceStrober, W. 1996. Obtaining human peripheral blood cells. In Current Protocols in Immunology ( J.E. Coligan, A.M. Kruisbeek, D.H. Margulies, E.M. Shevach, and W. Strober, eds.)pp. A.3.13 ‐ A.3.14. John Wiley & Sons, New York.
dc.identifier.citedreferenceWoffendin, C., Yang, Z.Y., Ranga, U., Xu, L., Yang, N.S., Sheehy, M.J., and Nabel, G.J. 1994. Nonviral and viral delivery of HIV protective gene into primary T cells. Proc. Natl. Acad. Sci. U.S.A. 91: 11581 ‐ 11585.
dc.identifier.citedreferenceWoffendin, C., Ranga, U., Yang, Z.Y., Xu, L., and Nabel, G.J. 1996. Expression of a protective gene prolongs survival of T cells in HIV infected patients. Proc. Natl. Acad. Sci. U.S.A. 93: 2889 ‐ 2894.
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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