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Probing DNA Structure with Hydroxyl Radicals
dc.contributor.authorTullius, Thomas D.
dc.date.accessioned2020-01-13T15:08:26Z
dc.date.available2020-01-13T15:08:26Z
dc.date.issued2001-12
dc.identifier.citationTullius, Thomas D. (2001). "Probing DNA Structure with Hydroxyl Radicals." Current Protocols in Nucleic Acid Chemistry 7(1): 6.7.1-6.7.8.
dc.identifier.issn1934-9270
dc.identifier.issn1934-9289
dc.identifier.urihttps://hdl.handle.net/2027.42/152721
dc.description.abstractThe hydroxyl radical is a useful probe for studying the shape of the surface of a DNA molecule. Using this technique, fine details of DNA structure can potentially be revealed. This unit describes how to use the hydroxyl radical to generate a random cleavage pattern at the surface of the molecule, separate the broken DNA strands by polyacrylamide gel electrophoresis, and analyze the cleavage pattern to give an image of the surface of the molecule.
dc.publisherWiley Periodicals, Inc.
dc.titleProbing DNA Structure with Hydroxyl Radicals
dc.typeArticle
dc.rights.robotsIndexNoFollow
dc.subject.hlbsecondlevelChemical Engineering
dc.subject.hlbsecondlevelChemistry
dc.subject.hlbsecondlevelPublic Health
dc.subject.hlbsecondlevelBiological Chemistry
dc.subject.hlbtoplevelScience
dc.subject.hlbtoplevelEngineering
dc.subject.hlbtoplevelHealth Sciences
dc.description.peerreviewedPeer Reviewed
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/152721/1/cpnc0607.pdf
dc.identifier.doi10.1002/0471142700.nc0607s07
dc.identifier.sourceCurrent Protocols in Nucleic Acid Chemistry
dc.identifier.citedreferenceBalasubramanian, B., Pogozelski, W.K., and Tullius, T.D. 1998. DNA strand breaking by the hydroxyl radical is governed by the accessible surface areas of the hydrogen atoms of the DNA backbone. Proc. Natl. Acad. Sci. U.S.A. 95: 9738 ‐ 9743.
dc.identifier.citedreferenceBurkhoff, A.M. and Tullius, T.D. 1987. The unusual conformation adopted by the adenine tracts in kinetoplast DNA. Cell 48: 935 ‐ 943.
dc.identifier.citedreferenceBurkhoff, A.M. and Tullius, T.D. 1988. Structural details of an adenine tract that does not cause DNA to bend. Nature 331: 455 ‐ 457.
dc.identifier.citedreferenceChurchill, M.E.A., Tullius, T.D., Kallenbach, N.R., and Seeman, N.C. 1988. A Holliday recombination intermediate is twofold symmetric. Proc. Natl. Acad. Sci. U.S.A. 85: 4653 ‐ 4656.
dc.identifier.citedreferenceKimball, A., Guo, Q., Lu, M., Cunningham, R.P., Kallenbach, N.R., Seeman, N.C., and Tullius, T.D. 1990. Construction and analysis of parallel and antiparallel Holliday junctions. J. Biol. Chem. 265: 6544 ‐ 6547.
dc.identifier.citedreferencePrice, M.A. and Tullius, T.D. 1992. Using the hydroxyl radical to probe DNA structure. Methods Enzymol. 212: 194 ‐ 219.
dc.identifier.citedreferencePrice, M.A. and Tullius, T.D. 1993. How the structure of an adenine tract depends on sequence context. A new model for the structure of T n A n DNA sequences. Biochemistry 32: 127 ‐ 136.
dc.identifier.citedreferenceShadle, S.E., Allen, D.F., Guo, H., Pogozelski, W.K., Bashkin, J.S., and Tullius, T.D. 1997. Quantitative analysis of electrophoresis data: Novel curve fitting methodology and its application to the determination of a protein‐DNA binding constant. Nucl. Acids Res. 25: 850 ‐ 861.
dc.identifier.citedreferenceShafer, G.E., Price, M.A., and Tullius, T.D. 1989. Use of the hydroxyl radical and gel electrophoresis to study DNA structure. Electrophoresis 10: 397 ‐ 404.
dc.identifier.citedreferenceUdenfriend, S., Clark, C.T., Axelrod, J., and Brodie, B.B. 1954. Ascorbic acid in aromatic hydroxylation. J. Biol. Chem. 208: 731 ‐ 739.
dc.identifier.citedreferencePrice and Tullius, 1992.See above.
dc.identifier.citedreferencehttp://people.bu.edu/tullius/GelExplorer_Manual.pdf
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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