Nonradioactive Analysis of Dynamic Protein Palmitoylation
dc.contributor.author | Martin, Brent R. | |
dc.date.accessioned | 2020-01-13T15:09:15Z | |
dc.date.available | 2020-01-13T15:09:15Z | |
dc.date.issued | 2013-08 | |
dc.identifier.citation | Martin, Brent R. (2013). "Nonradioactive Analysis of Dynamic Protein Palmitoylation." Current Protocols in Protein Science 73(1): 14.15.1-14.15.9. | |
dc.identifier.issn | 1934-3655 | |
dc.identifier.issn | 1934-3663 | |
dc.identifier.uri | https://hdl.handle.net/2027.42/152755 | |
dc.description.abstract | Methods to study protein S‐palmitoylation dynamics have previously relied on metabolic labeling with [14C]palmitate, which requires additional safety precautions and long exposures. Nonradioactive alkynyl palmitate analogs have been developed for in‐gel fluorescence detection and affinity purification. Cells metabolically labeled with the commercially available analog 17‐octadynoic acid are lysed and then combined with azide‐linked reporter tags for efficient conjugation by copper‐catalyzed click chemistry in phosphate buffer. This approach has been demonstrated to label hundreds of endogenous palmitoylated proteins and is compatible with traditional pulse‐chase methods. This protocol describes the reagents and procedures for labeling and detection of dynamic palmitoylation in mammalian cells. Curr. Protoc. Protein Sci. 73:14.15.1‐14.15.9. © 2013 by John Wiley & Sons, Inc. | |
dc.publisher | Wiley Periodicals, Inc. | |
dc.subject.other | post‐translational modifications | |
dc.subject.other | click chemistry | |
dc.subject.other | palmitoylation | |
dc.title | Nonradioactive Analysis of Dynamic Protein Palmitoylation | |
dc.type | Article | |
dc.rights.robots | IndexNoFollow | |
dc.subject.hlbsecondlevel | Public Health | |
dc.subject.hlbsecondlevel | Chemistry | |
dc.subject.hlbsecondlevel | Chemical Engineering | |
dc.subject.hlbsecondlevel | Biological Chemistry | |
dc.subject.hlbtoplevel | Engineering | |
dc.subject.hlbtoplevel | Science | |
dc.subject.hlbtoplevel | Health Sciences | |
dc.description.peerreviewed | Peer Reviewed | |
dc.description.bitstreamurl | https://deepblue.lib.umich.edu/bitstream/2027.42/152755/1/cpps1415.pdf | |
dc.identifier.doi | 10.1002/0471140864.ps1415s73 | |
dc.identifier.source | Current Protocols in Protein Science | |
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dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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