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Time‐Resolved Hydroxyl Radical Footprinting of RNA with X‐Rays
dc.contributor.authorWoodson, Sarah A.
dc.contributor.authorDeras, Michael L.
dc.contributor.authorBrenowitz, Michael
dc.date.accessioned2020-01-13T15:10:18Z
dc.date.available2020-01-13T15:10:18Z
dc.date.issued2001-09
dc.identifier.citationWoodson, Sarah A.; Deras, Michael L.; Brenowitz, Michael (2001). "Time‐Resolved Hydroxyl Radical Footprinting of RNA with X‐Rays." Current Protocols in Nucleic Acid Chemistry 6(1): 11.6.1-11.6.24.
dc.identifier.issn1934-9270
dc.identifier.issn1934-9289
dc.identifier.urihttps://hdl.handle.net/2027.42/152803
dc.description.abstractRNA tertiary structure and protein interactions can be detected by protection from hydroxyl radical cleavage. Generation of hydroxyl radicals with a synchrotron X‐ray beam provides analysis on a short timescale (50 msec to 100 sec), which enables the structures of folding intermediates or other transient conformational states to be determined. This unit provides detailed instructions on the use of the synchrotron beamline for hydroxyl radical footprinting.
dc.publisherWiley Periodicals, Inc.
dc.publisherPractical Applications.
dc.titleTime‐Resolved Hydroxyl Radical Footprinting of RNA with X‐Rays
dc.typeArticle
dc.rights.robotsIndexNoFollow
dc.subject.hlbsecondlevelBiological Chemistry
dc.subject.hlbsecondlevelChemical Engineering
dc.subject.hlbsecondlevelChemistry
dc.subject.hlbsecondlevelPublic Health
dc.subject.hlbtoplevelHealth Sciences
dc.subject.hlbtoplevelScience
dc.subject.hlbtoplevelEngineering
dc.description.peerreviewedPeer Reviewed
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/152803/1/cpnc1106.pdf
dc.identifier.doi10.1002/0471142700.nc1106s06
dc.identifier.sourceCurrent Protocols in Nucleic Acid Chemistry
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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