3′‐Modified Oligonucleotides and their Conjugates
dc.contributor.author | Lyttle, Matthew H. | |
dc.date.accessioned | 2020-01-13T15:10:34Z | |
dc.date.available | 2020-01-13T15:10:34Z | |
dc.date.issued | 2000-12 | |
dc.identifier.citation | Lyttle, Matthew H. (2000). "3′‐Modified Oligonucleotides and their Conjugates." Current Protocols in Nucleic Acid Chemistry 3(1): 4.6.1-4.6.8. | |
dc.identifier.issn | 1934-9270 | |
dc.identifier.issn | 1934-9289 | |
dc.identifier.uri | https://hdl.handle.net/2027.42/152815 | |
dc.description.abstract | Solid‐phase synthesis of 3′‐aminoalkyl, 3′‐thioalkyl, and 3′‐polyethyleneglycol are described. The first two are important as specific points of attachment for a large variety of reporter groups; the latter is important because of its increased cell membrane permeability, which aids in the development of antisense drugs. These protocols cover details of the synthetic organic chemistry needed to make each solid support, as well as DNA synthesis, workup, characterization, and conjugation. | |
dc.publisher | Wiley Periodicals, Inc. | |
dc.publisher | Pierce Chemical Company | |
dc.title | 3′‐Modified Oligonucleotides and their Conjugates | |
dc.type | Article | |
dc.rights.robots | IndexNoFollow | |
dc.subject.hlbsecondlevel | Biological Chemistry | |
dc.subject.hlbsecondlevel | Chemical Engineering | |
dc.subject.hlbsecondlevel | Chemistry | |
dc.subject.hlbsecondlevel | Public Health | |
dc.subject.hlbtoplevel | Science | |
dc.subject.hlbtoplevel | Engineering | |
dc.subject.hlbtoplevel | Health Sciences | |
dc.description.peerreviewed | Peer Reviewed | |
dc.description.bitstreamurl | https://deepblue.lib.umich.edu/bitstream/2027.42/152815/1/cpnc0406.pdf | |
dc.identifier.doi | 10.1002/0471142700.nc0406s03 | |
dc.identifier.source | Current Protocols in Nucleic Acid Chemistry | |
dc.identifier.citedreference | Gupta, K.C., Sharma, P., Kumar, P., and Sathyanarayana, S. 1991. A general method for the synthesis of 3′‐sulfhydryl and phosphate group containing oligonucleotides. Nucl. Acids Res. 19: 3019 ‐ 3025. | |
dc.identifier.citedreference | Lyttle, M.H., Adams, H., Hudson, D., and Cook, R.M. 1997. Versatile linker chemistry for synthesis of 3′‐modified DNA. Bioconjug. Chem. 8: 193 ‐ 198. | |
dc.identifier.citedreference | Stewart, J. and Young, J. 1984. Laboratory techniques in solid phase peptide synthesis. In Solid Phase Peptide Synthesis, pp. 105 ‐ 107. Pierce Chemical Company, Rockford, Ill. | |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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