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Engineering Terminal Disulfide Bonds Into DNA
dc.contributor.authorGlick, Gary D.
dc.date.accessioned2020-01-13T15:11:51Z
dc.date.available2020-01-13T15:11:51Z
dc.date.issued2003-06
dc.identifier.citationGlick, Gary D. (2003). "Engineering Terminal Disulfide Bonds Into DNA." Current Protocols in Nucleic Acid Chemistry 13(1): 5.7.1-5.7.13.
dc.identifier.issn1934-9270
dc.identifier.issn1934-9289
dc.identifier.urihttps://hdl.handle.net/2027.42/152862
dc.description.abstractThis protocol presents a simple and general means of modifying nucleic acids with disulfide cross‐links. These cross‐links serve as powerful tools to probe the structure, dynamics, thermodynamics, folding, and function of DNA and RNA, much in the way that cystine cross‐links have been used to study proteins. The chemistry described has been used to synthesize disulfide‐cross‐linked hairpins and duplexes, higher‐order structures such as triplexes, non‐ground‐state conformations, and tRNAs. since the cross‐links form quantitatively by mild air oxidation and do not purturb either secondary or tertiary structure, this modification should prove quite useful for the study of nucleic acids.
dc.publisherWiley Periodicals, Inc.
dc.publisherSpringer‐Verlag
dc.subject.otherdisulfide cross‐link
dc.subject.otherstructure
dc.subject.otherdynamics
dc.subject.othernucleic acid
dc.subject.otherstability
dc.titleEngineering Terminal Disulfide Bonds Into DNA
dc.typeArticle
dc.rights.robotsIndexNoFollow
dc.subject.hlbsecondlevelBiological Chemistry
dc.subject.hlbsecondlevelChemical Engineering
dc.subject.hlbsecondlevelChemistry
dc.subject.hlbsecondlevelPublic Health
dc.subject.hlbtoplevelHealth Sciences
dc.subject.hlbtoplevelScience
dc.subject.hlbtoplevelEngineering
dc.description.peerreviewedPeer Reviewed
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/152862/1/cpnc0507.pdf
dc.identifier.doi10.1002/0471142700.nc0507s13
dc.identifier.sourceCurrent Protocols in Nucleic Acid Chemistry
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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